Standard Curve (Sample)
(Fig.1. Human Receptor-type tyrosine-protein phosphatase F (PTPRF) Standard Curve.)
Human Receptor-Type Tyrosine-Protein Phosphatase F (PTPRF) ELISA Kit | PTPRF elisa kit
Human Receptor-Type Tyrosine-Protein Phosphatase F (PTPRF) ELISA Kit
Gene Names
PTPRF; LAR; BNAH2
Reactivity
Human
Synonyms
Receptor-Type Tyrosine-Protein Phosphatase F (PTPRF); N/A; Human Receptor-Type Tyrosine-Protein Phosphatase F (PTPRF) ELISA Kit; PTPRF; FLJ43335; FLJ45062; FLJ45567; LAR; LCA-homolog; OTTHUMP00000008683; OTTHUMP00000008684; leukocyte antigen-related (LAR) PTP receptor; leukocyte antigen-related tyrosine phosphatase; protein tyrosine phos; PTPRF elisa kit
Reactivity
Human
Specificity
Human Receptor-type tyrosine-protein phosphatase F (PTPRF) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPRF. No significant cross-reactivity or interference between Human PTPRF and analogues was observed.
Sequence Length
1552
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Receptor-type tyrosine-protein phosphatase F (PTPRF) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Receptor-type tyrosine-protein phosphatase F (PTPRF) Standard Curve.)
Related Product Information for PTPRF elisa kit
Description: This Human Receptor-type tyrosine-protein phosphatase F (PTPRF) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRF in samples. An antibody specific for PTPRF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTPRF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Receptor-type tyrosine-protein phosphatase F is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherens junctions, as well as in the control of beta-catenin signaling.
Background: Receptor-type tyrosine-protein phosphatase F is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherens junctions, as well as in the control of beta-catenin signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
protein tyrosine phosphatase, receptor type, F isoform 2 variant, partial
NCBI Official Synonym Full Names
protein tyrosine phosphatase receptor type F
NCBI Official Symbol
PTPRF
NCBI Official Synonym Symbols
LAR; BNAH2
NCBI Protein Information
receptor-type tyrosine-protein phosphatase F
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase F
UniProt Gene Name
PTPRF
UniProt Synonym Gene Names
LAR; LAR
UniProt Entry Name
PTPRF_HUMAN
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Product Notes
The Human PTPRF ptprf (Catalog #AAA331233) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331233 ELISA Kit recognizes Human PTPRF. It is sometimes possible for the material contained within the vial of "Receptor-Type Tyrosine-Protein Phosphatase F (PTPRF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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