Standard Curve (Sample)
(Fig.1. Human Regulator of G-protein signaling 19 (RGS19) Standard Curve.)
Human Regulator Of G-Protein Signaling 19 (RGS19) ELISA Kit | RGS19 elisa kit
Human Regulator Of G-Protein Signaling 19 (RGS19) ELISA Kit
Gene Names
RGS19; GAIP; RGSGAIP
Reactivity
Human
Synonyms
Regulator Of G-Protein Signaling 19 (RGS19); N/A; Human Regulator Of G-Protein Signaling 19 (RGS19) ELISA Kit; RGS19; GAIP; RGSGAIP; G alpha interacting protein; G protein signalling regulator 19; guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein; regulator of G-protein sig; RGS19 elisa kit
Reactivity
Human
Specificity
Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit has high sensitivity and excellent specificity for detection of Human RGS19. No significant cross-reactivity or interference between Human RGS19 and analogues was observed.
Sequence Length
217
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Regulator of G-protein signaling 19 (RGS19) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Regulator of G-protein signaling 19 (RGS19) Standard Curve.)
Related Product Information for RGS19 elisa kit
Description: This Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit employs a two-site sandwich ELISA to quantitate RGS19 in samples. An antibody specific for RGS19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RGS19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sequence analysis predicted that GAIP is a 24.6-kD, hydrophilic protein that lacks a transmembrane domain and contains multiple potential phosphorylation sites. GAIP shares approximately 31% amino acid identity with regulator of G protein signaling-1 (RGS1) and -2 (RGS2); the 125-amino acid core domains of GAIP, RGS1, and RGS2 share approximately 64% homology. Northern blot analysis detected a 1.6-kb GAIP transcript in lung, placenta, liver, heart, and pancreas, with almost no expression detected in brain, skeletal muscle, and kidney. Yeast 2-hybrid binding analyses showed that through its core domain, GAIP interacts strongly with GNAI3 and weakly with GNAI2, but does not interact with GNA11.
Background: Sequence analysis predicted that GAIP is a 24.6-kD, hydrophilic protein that lacks a transmembrane domain and contains multiple potential phosphorylation sites. GAIP shares approximately 31% amino acid identity with regulator of G protein signaling-1 (RGS1) and -2 (RGS2); the 125-amino acid core domains of GAIP, RGS1, and RGS2 share approximately 64% homology. Northern blot analysis detected a 1.6-kb GAIP transcript in lung, placenta, liver, heart, and pancreas, with almost no expression detected in brain, skeletal muscle, and kidney. Yeast 2-hybrid binding analyses showed that through its core domain, GAIP interacts strongly with GNAI3 and weakly with GNAI2, but does not interact with GNA11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
regulator of G-protein signaling 19
NCBI Official Synonym Full Names
regulator of G protein signaling 19
NCBI Official Symbol
RGS19
NCBI Official Synonym Symbols
GAIP; RGSGAIP
NCBI Protein Information
regulator of G-protein signaling 19
UniProt Protein Name
Regulator of G-protein signaling 19
UniProt Gene Name
RGS19
UniProt Synonym Gene Names
GAIP; GNAI3IP; RGS19; GAIP
UniProt Entry Name
RGS19_HUMAN
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Product Notes
The Human RGS19 rgs19 (Catalog #AAA330999) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330999 ELISA Kit recognizes Human RGS19. It is sometimes possible for the material contained within the vial of "Regulator Of G-Protein Signaling 19 (RGS19), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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