Standard Curve (Sample)
(Fig.1. Human Reticulocalbin-2 (RCN2) Standard Curve.)
Human Reticulocalbin-2 (RCN2) ELISA Kit | RCN2 elisa kit
Human Reticulocalbin-2 (RCN2) ELISA Kit
Gene Names
RCN2; E6BP; ERC55; ERC-55; TCBP49
Reactivity
Human
Synonyms
Reticulocalbin-2 (RCN2); N/A; Human Reticulocalbin-2 (RCN2) ELISA Kit; RCN2; E6BP; ERC-55; ERC55; TCBP49; reticulocalbin 2; EF-hand calcium binding domain (endoplasmic reticulum calcium-binding protein; 55kD); RCN2 elisa kit
Reactivity
Human
Specificity
Human Reticulocalbin-2 (RCN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RCN2. No significant cross-reactivity or interference between Human RCN2 and analogues was observed.
Sequence Length
317
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Reticulocalbin-2 (RCN2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Reticulocalbin-2 (RCN2) Standard Curve.)
Related Product Information for RCN2 elisa kit
Description: This Human Reticulocalbin-2 (RCN2) ELISA Kit employs a two-site sandwich ELISA to quantitate RCN2 in samples. An antibody specific for RCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Reticulocalbin 2 is a calcium-binding protein located in the lumen of the ER, contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. The predicted 317-amino acid RCN2 protein contains an N-terminal signal sequence, 6 copies of the EF-hand motif, and a C-terminal His-Asp-Glu-Leu (HDEL) sequence, which was originally defined as an ER retention motif in yeast proteins. Immunocytolocalization and cell fractionation studies demonstrated that RCN2 is a resident ER protein; the HDEL motif is required for its ER retention. RCN2 was detected by antibodies in all cell lines tested. It binds calcium in vitro. By Western blotting, in vitro translated RCN2 protein has a molecular mass of 55 kD, leading the authors to designate it the 'endoplasmic reticulum calcium-binding protein of 55 kD' (ERC55).
Background: Reticulocalbin 2 is a calcium-binding protein located in the lumen of the ER, contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. The predicted 317-amino acid RCN2 protein contains an N-terminal signal sequence, 6 copies of the EF-hand motif, and a C-terminal His-Asp-Glu-Leu (HDEL) sequence, which was originally defined as an ER retention motif in yeast proteins. Immunocytolocalization and cell fractionation studies demonstrated that RCN2 is a resident ER protein; the HDEL motif is required for its ER retention. RCN2 was detected by antibodies in all cell lines tested. It binds calcium in vitro. By Western blotting, in vitro translated RCN2 protein has a molecular mass of 55 kD, leading the authors to designate it the 'endoplasmic reticulum calcium-binding protein of 55 kD' (ERC55).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Reticulocalbin-2
NCBI Official Synonym Full Names
reticulocalbin 2
NCBI Official Symbol
RCN2
NCBI Official Synonym Symbols
E6BP; ERC55; ERC-55; TCBP49
NCBI Protein Information
reticulocalbin-2
UniProt Protein Name
Reticulocalbin-2
UniProt Gene Name
RCN2
UniProt Synonym Gene Names
ERC55; E6BP
UniProt Entry Name
RCN2_HUMAN
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Product Notes
The Human RCN2 rcn2 (Catalog #AAA331012) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331012 ELISA Kit recognizes Human RCN2. It is sometimes possible for the material contained within the vial of "Reticulocalbin-2 (RCN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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