Human Retinol Binding Protein 1, Cellular (RBP1) ELISA Kit | RBP1 elisa kit

Human Retinol Binding Protein 1, Cellular (RBP1) ELISA Kit

Cat. Num. AAA20695

• Gene Names: RBP1; CRBP; RBPC; CRBP1; CRBPI; CRABP-I
• Reactivity: Human
• Synonyms: Retinol Binding Protein 1, Cellular (RBP1); N/A; Human Retinol Binding Protein 1, Cellular (RBP1) ELISA Kit; RBP-1; CRABP-I; CRBP; CRBP1; RBPC; Cellular retinol-binding protein I; RBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RASGRP2. No significant cross-reactivity or interference between RASGRP2 and analogues was observed.
• Sequence Length: 157
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RASGRP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RASGRP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for RBP1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RASGRP2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to RASGRP2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RASGRP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RASGRP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RASGRP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RBP1 elisa kit

Signal Transduction

References

Comprehensive Profiling of Secreted Factors in the Cerebrospinal Fluid of Moyamoya Disease Patients; K Abhinav

NCBI and Uniprot Product Information

• NCBI GI #: 195976809
• NCBI GeneID: 5947
• NCBI Accession #: NP_001124464.1
• NCBI GenBank Nucleotide #: NM_001130992.1
• UniProt Accession #: P09455; Q6FGX8; A8K2Q0; B7Z7A0; E7EWV0; F2Z2F2
• Molecular Weight: 17,523 Da
• NCBI Official Full Name: retinol-binding protein 1 isoform b
• NCBI Official Synonym Full Names: retinol binding protein 1, cellular
• NCBI Official Symbol: RBP1
• NCBI Official Synonym Symbols: CRBP; RBPC; CRBP1; CRBPI; CRABP-I
• NCBI Protein Information: retinol-binding protein 1
• UniProt Protein Name: Retinol-binding protein 1
• UniProt Gene Name: RBP1
• UniProt Synonym Gene Names: CRBP1; CRBP; CRBP-I
• UniProt Entry Name: RET1_HUMAN