Standard Curve (Sample)
Human Reverse T3 (rT3) ELISA Kit | rT3 elisa kit
Reverse T3 (rT3) ELISA Kit
Reactivity
Human
Synonyms
Reverse T3 (rT3); N/A; Reverse T3 (rT3) ELISA Kit; rT3 elisa kit
Reactivity
Human
Specificity
The following compounds were tested for cross-reactivity using the Abraham method with angiotensin-I cross reacti.n g at 100'*0 :
Assay Type
Quantitative Competitive
Samples
Human serum and plasma by an ELISA (Enzyme-Linked Immunosorbent Assay)
Preparation and Storage
Store at 2 - 8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for rT3 elisa kit
Principle of the Assay: The rT3 ELISA is a two-step competitive immunoassay. In the first incubation step, competition occurs between rT3 present in calibrators, controls, specimen samples and a biotin-labelled antigen (biotin conjugate) for a limited number of anti-rT3 antibody binding sites on the microplate wells. Excess and unbound materials are removed by a washing step. In the second incubation step, streptavidin-HRP (streptavidin HRP conjugate) is added, which binds specifically to any bound biotin conjugate. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of rT3 present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of rT3 in specimen samples and controls can be directly read.
Intended Uses: For the quantitative measurement of Reverse Triiodothyronine (rT3) in human serum and plasma by an ELISA (Enzyme-Linked Immunosorbent Assay).
Intended Uses: For the quantitative measurement of Reverse Triiodothyronine (rT3) in human serum and plasma by an ELISA (Enzyme-Linked Immunosorbent Assay).
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Product Notes
The Human rT3 (Catalog #AAA72679) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA72679 ELISA Kit recognizes Human rT3. It is sometimes possible for the material contained within the vial of "Reverse T3 (rT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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