Standard Curve (Sample)
(Fig.1. Human Ribonucleases P/MRP protein subunit POP1 (POP1) Standard Curve.)
Human Ribonucleases P/MRP Protein Subunit POP1 (POP1) ELISA Kit | POP1 elisa kit
Human Ribonucleases P/MRP Protein Subunit POP1 (POP1) ELISA Kit
Gene Names
POP1; ANXD2
Reactivity
Human
Synonyms
Ribonucleases P/MRP Protein Subunit POP1 (POP1); N/A; Human Ribonucleases P/MRP Protein Subunit POP1 (POP1) ELISA Kit; POP1; KIAA0061; MGC17365; processing of precursor 1; processing of precursors 1; POP1 elisa kit
Reactivity
Human
Specificity
Human Ribonucleases P/MRP protein subunit POP1 (POP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human POP1. No significant cross-reactivity or interference between Human POP1 and analogues was observed.
Sequence Length
3269
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Ribonucleases P/MRP protein subunit POP1 (POP1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Ribonucleases P/MRP protein subunit POP1 (POP1) Standard Curve.)
Related Product Information for POP1 elisa kit
Description: This Human Ribonucleases P/MRP protein subunit POP1 (POP1) ELISA Kit employs a two-site sandwich ELISA to quantitate POP1 in samples. An antibody specific for POP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: POP1 is a protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. This protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing.Human POP1 encodes a predicted 1,024-amino acid protein that has a pI of 9.86. On Northern blots, human POP1 is expressed as a major 4.1-kb and minor 6-kb transcript, perhaps due to alternative polyadenylation signals. Anti-POP1 antibodies recognize a doublet of approximately 115 kD on Western blots. In HeLa cells, human POP1 localizes to the nucleus and strongly accumulates in the nucleolus. In co-immunoprecipitation studies, POP1 was associated with both RNase P and RNase MRP snRNAs, and with RNase P enzymatic activity.
Background: POP1 is a protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. This protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing.Human POP1 encodes a predicted 1,024-amino acid protein that has a pI of 9.86. On Northern blots, human POP1 is expressed as a major 4.1-kb and minor 6-kb transcript, perhaps due to alternative polyadenylation signals. Anti-POP1 antibodies recognize a doublet of approximately 115 kD on Western blots. In HeLa cells, human POP1 localizes to the nucleus and strongly accumulates in the nucleolus. In co-immunoprecipitation studies, POP1 was associated with both RNase P and RNase MRP snRNAs, and with RNase P enzymatic activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Homo sapiens processing of 1, ribonuclease P/MRP subunit (S. cerevisiae), mRNA
NCBI Official Synonym Full Names
POP1 homolog, ribonuclease P/MRP subunit
NCBI Official Symbol
POP1
NCBI Official Synonym Symbols
ANXD2
NCBI Protein Information
ribonucleases P/MRP protein subunit POP1
UniProt Protein Name
Ribonucleases P/MRP protein subunit POP1
UniProt Gene Name
POP1
UniProt Synonym Gene Names
KIAA0061; hPOP1
UniProt Entry Name
POP1_HUMAN
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Product Notes
The Human POP1 pop1 (Catalog #AAA331375) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331375 ELISA Kit recognizes Human POP1. It is sometimes possible for the material contained within the vial of "Ribonucleases P/MRP Protein Subunit POP1 (POP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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