Standard Curve (Sample)
(Fig.1. Human RNA-binding protein 24 (RBM24) Standard Curve.)
Human RNA-Binding Protein 24 (RBM24) ELISA Kit | RBM24 elisa kit
Human RNA-Binding Protein 24 (RBM24) ELISA Kit
Gene Names
RBM24; RNPC6; dJ259A10.1
Reactivity
Human
Synonyms
RNA-Binding Protein 24 (RBM24); N/A; Human RNA-Binding Protein 24 (RBM24) ELISA Kit; RBM24; FLJ26355; FLJ30829; FLJ37697; RNPC6; dJ259A10.1; RNA-binding region (RNP1; RRM) containing 6; RBM24 elisa kit
Reactivity
Human
Specificity
Human RNA-binding protein 24 (RBM24) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBM24. No significant cross-reactivity or interference between Human RBM24 and analogues was observed.
Sequence Length
236
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human RNA-binding protein 24 (RBM24) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human RNA-binding protein 24 (RBM24) Standard Curve.)
Related Product Information for RBM24 elisa kit
Description: This Human RNA-binding protein 24 (RBM24) ELISA Kit employs a two-site sandwich ELISA to quantitate RBM24 in samples. An antibody specific for RBM24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBM24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RBM24 is restricted to the lateral and ventral mesoderm during gastrulation and then localized to the somitic mesoderm, in a similar pattern as XMyoD gene. A 0.65kb X. tropicalis RBM24 regulatory region contains multiple E boxes (CANNTG), which are potential binding sites for MyoD and other bHLH proteins. Knockdown of Seb4 produces similar effects as those obtained by the dominant negative MyoD mutant, indicating that it is required for the expression of myogenic genes and myogenesis in the embryo. In cultured ectodermal explants, although overexpression of Seb4 has no obvious effect on myogenesis, knockdown of Seb4 inhibits the expression of myogenic genes and myogenesis induced by MyoD. Seb4/RBM24 is a direct target of MyoD and is required for myogenesis during Xenopus early development.
Background: RBM24 is restricted to the lateral and ventral mesoderm during gastrulation and then localized to the somitic mesoderm, in a similar pattern as XMyoD gene. A 0.65kb X. tropicalis RBM24 regulatory region contains multiple E boxes (CANNTG), which are potential binding sites for MyoD and other bHLH proteins. Knockdown of Seb4 produces similar effects as those obtained by the dominant negative MyoD mutant, indicating that it is required for the expression of myogenic genes and myogenesis in the embryo. In cultured ectodermal explants, although overexpression of Seb4 has no obvious effect on myogenesis, knockdown of Seb4 inhibits the expression of myogenic genes and myogenesis induced by MyoD. Seb4/RBM24 is a direct target of MyoD and is required for myogenesis during Xenopus early development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
RNA-binding protein 24 isoform 1
NCBI Official Synonym Full Names
RNA binding motif protein 24
NCBI Official Symbol
RBM24
NCBI Official Synonym Symbols
RNPC6; dJ259A10.1
NCBI Protein Information
RNA-binding protein 24
UniProt Protein Name
RNA-binding protein 24
UniProt Gene Name
RBM24
UniProt Synonym Gene Names
RNPC6
UniProt Entry Name
RBM24_HUMAN
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Product Notes
The Human RBM24 rbm24 (Catalog #AAA331019) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331019 ELISA Kit recognizes Human RBM24. It is sometimes possible for the material contained within the vial of "RNA-Binding Protein 24 (RBM24), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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