Standard Curve (Sample)
Human Rotavirus IgG ELISA Kit
Human Anti-Rotavirus IgG ELISA Kit
Reactivity
Human
Synonyms
Rotavirus IgG; N/A; Human Anti-Rotavirus IgG ELISA Kit; Rotavirus IgG elisa kit
Reactivity
Human
Specificity
Human Anti-Rotavirus IgG ELISA Kit has high sensitivity and excellent specificity for detection of Human Anti-Rotavirus IgG. No significant cross-reactivity or interference between Human Anti-Rotavirus IgG and analogues was observed.
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
1.5 ng/L - 24 ng/L
Sensitivity
< 0.1ng/L
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Rotavirus IgG elisa kit
Background: Rotavirus is one of the main pathogens that cause diarrhea in infants and young children. It mainly infects intestinal epithelial cells, causing cell damage and causing diarrhea. Rotavirus is prevalent in summer, autumn and winter every year. The route of infection is the fecal-oral route, and the clinical manifestation is acute gastroenteritis with osmotic diarrhea.
Principle of the Assay: Human Anti-Rotavirus IgG ELISA Kit quantitate Human Anti-Rotavirus IgG in samples. An antigen has been pre-coated onto a microplate. Standards or test samples are added to the wells, incubated and then washed. HRP-conjugated antigen is then added and incubated. The plate is washed once more and the chromogen solution is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
Principle of the Assay: Human Anti-Rotavirus IgG ELISA Kit quantitate Human Anti-Rotavirus IgG in samples. An antigen has been pre-coated onto a microplate. Standards or test samples are added to the wells, incubated and then washed. HRP-conjugated antigen is then added and incubated. The plate is washed once more and the chromogen solution is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
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Product Notes
The Human Rotavirus IgG (Catalog #AAA31512) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA31512 ELISA Kit recognizes Human Rotavirus IgG. It is sometimes possible for the material contained within the vial of "Rotavirus IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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