Standard Curve (Sample)
Human sCD40L ELISA Kit | sCD40L elisa kit
Human sCD40L ELISA Kit
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Reactivity
Human
Synonyms
sCD40L; N/A; Human sCD40L ELISA Kit; sCD40L elisa kit
Reactivity
Human
Specificity
This assay recognizes both recombinant and natural human sCD40L. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity. No significant cross-reactivity was observed with the following: Recombinant human: ANG AR BDNF CD4 CD40 CNTF CT-1 CTLA-4 Epo Fas CDNF GITR OPG OSM Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sCD40L and all the analogues, therefore, cross reaction may still exist.
Sequence Length
261
Assay Type
Quantitative Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
< 31.25pg/mL
Intra-assay Precision
Intra-plate Precision The three samples with known concentrations were repeated 20 times on the same board to calculate the coefficient of variation (CV) for the concentrations. Intra-Assay: CV<10%
Inter-assay Precision
Inter-plate Precision The coefficient of variation (CV) of three known concentrations was calculated by 20 tests of two kits on each of the three samples. Inter-Assay: CV<15%
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for sCD40L elisa kit
Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of sCD40L in human serum, plasma, cell culture supernatants and other biological fluids.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human sCD40L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD40L present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated detection antibody specific for human sCD40L is added to the wells. After incubating, an enzyme-linked streptavidin is added and binds to biotin. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD40L bound in the initial step. The color development is stopped, and the absorbance is measured.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human sCD40L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD40L present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated detection antibody specific for human sCD40L is added to the wells. After incubating, an enzyme-linked streptavidin is added and binds to biotin. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD40L bound in the initial step. The color development is stopped, and the absorbance is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,274 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
NCBI Protein Information
CD40 ligand
UniProt Protein Name
CD40 ligand
UniProt Gene Name
CD40LG
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP
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Product Notes
The Human sCD40L cd40lg (Catalog #AAA281333) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281333 ELISA Kit recognizes Human sCD40L. It is sometimes possible for the material contained within the vial of "sCD40L, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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