Standard Curve (Sample)
(Fig.1. Human Secreted frizzled-related protein 2 (SFRP2) Standard Curve.)
Human Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit | SFRP2 elisa kit
Human Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Human
Synonyms
Secreted Frizzled-Related Protein 2 (SFRP2); N/A; Human Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit; SFRP2; FRP-2; SARP1; SDF-5; secreted apoptosis related protein 1; SFRP2 elisa kit
Reactivity
Human
Specificity
Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFRP2. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed.
Sequence Length
295
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Secreted frizzled-related protein 2 (SFRP2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Secreted frizzled-related protein 2 (SFRP2) Standard Curve.)
Related Product Information for SFRP2 elisa kit
Description: This Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP2 in samples. An antibody specific for SFRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SFRP2 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. The partial human SARP1 cDNA encodes a polypeptide that is 95% identical to mouse Sarp1. When expressed in a breast adenocarcinoma cell line, mouse SARP1 and human SARP2 exhibited opposite effects on cell sensitivity to proapoptotic stimuli. Whereas cells with SARP1 had higher resistance, cells expressing SARP2 were sensitized to apoptosis induced by tumor necrosis factor and ceramide. Expression of SARP1 or SARP2 modified the intracellular levels of beta-catenin, an indicator of Wnt-frizzled protein interaction and signal transduction, suggesting that SARPs interfere with the Wnt-frizzled signaling pathway.
Background: SFRP2 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. The partial human SARP1 cDNA encodes a polypeptide that is 95% identical to mouse Sarp1. When expressed in a breast adenocarcinoma cell line, mouse SARP1 and human SARP2 exhibited opposite effects on cell sensitivity to proapoptotic stimuli. Whereas cells with SARP1 had higher resistance, cells expressing SARP2 were sensitized to apoptosis induced by tumor necrosis factor and ceramide. Expression of SARP1 or SARP2 modified the intracellular levels of beta-catenin, an indicator of Wnt-frizzled protein interaction and signal transduction, suggesting that SARPs interfere with the Wnt-frizzled signaling pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
secreted frizzled-related protein 2
NCBI Official Synonym Full Names
secreted frizzled related protein 2
NCBI Official Symbol
SFRP2
NCBI Official Synonym Symbols
FRP-2; SARP1; SDF-5
NCBI Protein Information
secreted frizzled-related protein 2
UniProt Protein Name
Secreted frizzled-related protein 2
UniProt Gene Name
SFRP2
UniProt Synonym Gene Names
FRP2; SARP1; FRP-2; sFRP-2; SARP-1
UniProt Entry Name
SFRP2_HUMAN
Similar Products
Product Notes
The Human SFRP2 sfrp2 (Catalog #AAA330891) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330891 ELISA Kit recognizes Human SFRP2. It is sometimes possible for the material contained within the vial of "Secreted Frizzled-Related Protein 2 (SFRP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.