Human Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit | SFRP4 elisa kit
Human Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit
Gene Names
SFRP4; PYL; FRP-4; FRPHE; FRZB-2; sFRP-4
Reactivity
Human
Synonyms
Secreted Frizzled Related Protein 4 (SFRP4); N/A; Human Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit; SFRP4 elisa kit
Reactivity
Human
Sequence Length
346
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.05-15ng/ml
Sensitivity
0.024ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for SFRP4 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Secreted Frizzled Related Protein 4 (also known as SFRP4) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SFRP4 antibody. SFRP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SFRP4 Antibody is added and binds to SFRP4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFRP4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SFRP4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SFRP4 antibody. SFRP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SFRP4 Antibody is added and binds to SFRP4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFRP4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SFRP4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,827 Da
NCBI Official Full Name
secreted frizzled-related protein 4
NCBI Official Synonym Full Names
secreted frizzled related protein 4
NCBI Official Symbol
SFRP4
NCBI Official Synonym Symbols
PYL; FRP-4; FRPHE; FRZB-2; sFRP-4
NCBI Protein Information
secreted frizzled-related protein 4
UniProt Protein Name
Secreted frizzled-related protein 4
UniProt Gene Name
SFRP4
UniProt Synonym Gene Names
FRPHE; sFRP-4; FrpHE