Reactivity
Human
Sequence Length
751
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
20-3500ng/L
Sensitivity
10.23ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for SEMA3C elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SEMA3C antibody. SEMA3C present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA3C Antibody is added and binds to SEMA3C in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA3C antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA3C. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Semaphorin 3C (also known as SEMA3C) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Semaphorin 3C (also known as SEMA3C) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,255 Da
NCBI Official Full Name
semaphorin-3C isoform 2
NCBI Official Synonym Full Names
semaphorin 3C
NCBI Official Symbol
SEMA3C
NCBI Official Synonym Symbols
SemE; SEMAE
NCBI Protein Information
semaphorin-3C
UniProt Protein Name
Semaphorin-3C
UniProt Gene Name
SEMA3C
UniProt Synonym Gene Names
SEMAE; Sema E