Human Semaphorin 4A ELISA Kit | SEMA4A elisa kit
Human Semaphorin 4A ELISA Kit
Gene Names
SEMA4A; RP35; SEMB; SEMAB; CORD10; RP11-54H19.2
Reactivity
Human
Synonyms
Semaphorin 4A; N/A; Human Semaphorin 4A ELISA Kit; SEMA4A elisa kit
Reactivity
Human
Assay Type
sandwich
Samples
cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<50pg/ml
Detection Target
Semaphorin 4A
Immunogen Sequence
369
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Related Product Information for SEMA4A elisa kit
This Human Semaphorin 4A ELISA Kit is intended for quantitative detection of human SEMA4A in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This human SEMA4A ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SEMA4A has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G33-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEMA4A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SEMA4A amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Semaphorin-4A is a protein that in humans is encoded by the SEMA4A gene. This gene is mapped to 1q22. SEMA4A encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
This human SEMA4A ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SEMA4A has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G33-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEMA4A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SEMA4A amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Semaphorin-4A is a protein that in humans is encoded by the SEMA4A gene. This gene is mapped to 1q22. SEMA4A encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,574 Da
NCBI Official Full Name
semaphorin-4A isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
NCBI Official Symbol
SEMA4A
NCBI Official Synonym Symbols
RP35; SEMB; SEMAB; CORD10; RP11-54H19.2
NCBI Protein Information
semaphorin-4A; sema B; semaphorin-B
UniProt Protein Name
Semaphorin-4A
UniProt Gene Name
SEMA4A
UniProt Synonym Gene Names
SEMAB; SEMB; Sema B
UniProt Entry Name
SEM4A_HUMAN
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Product Notes
The Human SEMA4A sema4a (Catalog #AAA266528) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA266528 ELISA Kit recognizes Human SEMA4A. It is sometimes possible for the material contained within the vial of "Semaphorin 4A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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