Human Serglycin (SRGN) ELISA Kit | SRGN elisa kit

Human Serglycin (SRGN) ELISA Kit

Cat. Num. AAA190239

• Gene Names: SRGN; PPG; PRG; PRG1
• Reactivity: Human
• Synonyms: Serglycin (SRGN); N/A; Human Serglycin (SRGN) ELISA Kit; SRGN ELISA kit (Human); Serglycin; PRG; PPG; PRG1; proteoglycan 1, secretory granule; SRGN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serglycin (SRGN). No significant cross-reactivity or interference between Serglycin (SRGN) and analogues was observed.; Reactive with Human SRGN/Serglycin
• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and other biological fluids
• Detection Range: 123.5-10,000pg/mL
• Sensitivity: 51.5pg/mL
• Sample Size: 50uL
• Detection Method: Colormetric
• Featured Series: CE kit

• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serglycin (SRGN) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serglycin (SRGN) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%, Inter-Assay: CV<12%
• Function: Detects small molecule
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 50ul standard or sample to each well. And then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 degree C; 3. Aspirate and wash 3 times; 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 5. Aspirate and wash 5 times; 6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 7. Add 50ul Stop Solution. Read at 450 nm immediately.
• Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Serglycin (SRGN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Serglycin (SRGN) and unlabeled Serglycin (SRGN) (Standards or samples) with the pre-coated antibody specific to Serglycin (SRGN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Serglycin (SRGN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Serglycin (SRGN) in the sample.
• Research Area: Infection immunity
• Preparation and Storage: Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 45935371
• NCBI GeneID: 5552
• NCBI Accession #: NP_002718.2
• NCBI GenBank Nucleotide #: NM_002727.3
• UniProt Accession #: P10124; Q5VW06; B2R4L7
• NCBI Official Full Name: serglycin isoform 1
• NCBI Official Synonym Full Names: serglycin
• NCBI Official Symbol: SRGN
• NCBI Official Synonym Symbols: PPG; PRG; PRG1
• NCBI Protein Information: serglycin
• UniProt Protein Name: Serglycin
• UniProt Gene Name: SRGN
• UniProt Synonym Gene Names: PRG; PRG1; P.PG
• UniProt Entry Name: SRGN_HUMAN