Standard Curve (Sample)
(Fig.1. Human Sex hormone-binding globulin (SHBG) Standard Curve.)
Human Sex Hormone-Binding Globulin (SHBG) ELISA Kit | SHBG elisa kit
Human Sex Hormone-Binding Globulin (SHBG) ELISA Kit
Gene Names
SHBG; ABP; SBP; TEBG
Reactivity
Human
Synonyms
Sex Hormone-Binding Globulin (SHBG); N/A; Human Sex Hormone-Binding Globulin (SHBG) ELISA Kit; SHBG; ABP; MGC126834; MGC138391; SBP; TEBG; androgen binding protein; sex steroid-binding protein; testis-specific androgen-binding protein; testosterone-binding beta-globulin; testosterone-estradiol-binding; SHBG elisa kit
Reactivity
Human
Specificity
Human Sex hormone-binding globulin (SHBG) ELISA Kit has high sensitivity and excellent specificity for detection of Human SHBG. No significant cross-reactivity or interference between Human SHBG and analogues was observed.
Sequence Length
402
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Sex hormone-binding globulin (SHBG) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Sex hormone-binding globulin (SHBG) Standard Curve.)
Related Product Information for SHBG elisa kit
Description: This Human Sex hormone-binding globulin (SHBG) ELISA Kit employs a two-site sandwich ELISA to quantitate SHBG in samples. An antibody specific for SHBG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SHBG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SHBG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SHBG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SHBG is a glycoprotein that binds to sex hormones, specifically testosterone and estradiol. Other steroid hormones such as progesterone, cortisol, and other corticosteroids are bound by transcortin. These sex hormones circulate in the bloodstream, bound mostly to SHBG and to some degree bound to serum albumin. Only a small fraction is unbound, or "free," and thus biologically active and able to enter a cell and activate its receptor. The SHBG inhibits the function of these hormones. Thus bioavailability of sex hormones is influenced by the level of SHBG. SHBG is produced by the liver cells and is released into the bloodstream. Other sites that produce SHBG are the brain, uterus, and placenta and vagina. In addition SHBG is produced by the testes; testes-produced SHBG is also called androgen-binding protein.
Background: SHBG is a glycoprotein that binds to sex hormones, specifically testosterone and estradiol. Other steroid hormones such as progesterone, cortisol, and other corticosteroids are bound by transcortin. These sex hormones circulate in the bloodstream, bound mostly to SHBG and to some degree bound to serum albumin. Only a small fraction is unbound, or "free," and thus biologically active and able to enter a cell and activate its receptor. The SHBG inhibits the function of these hormones. Thus bioavailability of sex hormones is influenced by the level of SHBG. SHBG is produced by the liver cells and is released into the bloodstream. Other sites that produce SHBG are the brain, uterus, and placenta and vagina. In addition SHBG is produced by the testes; testes-produced SHBG is also called androgen-binding protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sex hormone-binding globulin isoform 1
NCBI Official Synonym Full Names
sex hormone binding globulin
NCBI Official Symbol
SHBG
NCBI Official Synonym Symbols
ABP; SBP; TEBG
NCBI Protein Information
sex hormone-binding globulin
UniProt Protein Name
Sex hormone-binding globulin
UniProt Gene Name
SHBG
UniProt Synonym Gene Names
SHBG; SBP; ABP; TeBG
UniProt Entry Name
SHBG_HUMAN
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Product Notes
The Human SHBG shbg (Catalog #AAA330878) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330878 ELISA Kit recognizes Human SHBG. It is sometimes possible for the material contained within the vial of "Sex Hormone-Binding Globulin (SHBG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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