Standard Curve (Sample)
(Fig.1. Human Sialoadhesin (SIGLEC1) Standard Curve.)
Human Sialoadhesin (SIGLEC1) ELISA Kit | SIGLEC1 elisa kit
Human Sialoadhesin (SIGLEC1) ELISA Kit
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Synonyms
Sialoadhesin (SIGLEC1); N/A; Human Sialoadhesin (SIGLEC1) ELISA Kit; SIGLEC1; CD169; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; SIGLEC-1; SN; dJ1009E24.1; sialic acid-binding immunoglobulin-like lectin 1; sialoadhesin; SIGLEC1 elisa kit
Reactivity
Human
Specificity
Human Sialoadhesin (SIGLEC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIGLEC1. No significant cross-reactivity or interference between Human SIGLEC1 and analogues was observed.
Sequence Length
1649
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Sialoadhesin (SIGLEC1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Sialoadhesin (SIGLEC1) Standard Curve.)
Related Product Information for SIGLEC1 elisa kit
Description: This Human Sialoadhesin (SIGLEC1) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC1 in samples. An antibody specific for SIGLEC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIGLEC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sialoadhesin is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. It is defined as an I-type lectin, since it contains 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains), and thus also belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. Alternate names for sialoadhesin include siglec-1 and CD169 (cluster of differentiation 169). Sialoadhesin predominately binds neutrophils, but can also bind monocytes, natural killer cells, B cells and a subset of cytotoxic T cells by interacting with sialic acid molecules in the ligands on their surfaces.
Background: Sialoadhesin is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. It is defined as an I-type lectin, since it contains 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains), and thus also belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. Alternate names for sialoadhesin include siglec-1 and CD169 (cluster of differentiation 169). Sialoadhesin predominately binds neutrophils, but can also bind monocytes, natural killer cells, B cells and a subset of cytotoxic T cells by interacting with sialic acid molecules in the ligands on their surfaces.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
sialic acid binding Ig-like lectin 1, sialoadhesin, isoform CRA_b, partial
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 1
NCBI Official Symbol
SIGLEC1
NCBI Official Synonym Symbols
SN; CD169; SIGLEC-1
NCBI Protein Information
sialoadhesin
UniProt Protein Name
Sialoadhesin
UniProt Gene Name
SIGLEC1
UniProt Synonym Gene Names
SN; Siglec-1
UniProt Entry Name
SN_HUMAN
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Product Notes
The Human SIGLEC1 siglec1 (Catalog #AAA330873) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330873 ELISA Kit recognizes Human SIGLEC1. It is sometimes possible for the material contained within the vial of "Sialoadhesin (SIGLEC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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