Standard Curve (Sample)
(Fig.1. Human Signal transducer and activator of transcription 1 (STAT1) Standard Curve.)
Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit | STAT1 elisa kit
Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human
Synonyms
Signal Transducer And Activator Of Transcription 1 (STAT1); N/A; Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit; STAT1; DKFZp686B04100; ISGF-3; STAT91; OTTHUMP00000205845; signal transducer and activator of transcription 1; signal transducer and activator of transcription-1; transcription factor ISGF-3 components p91/p8; STAT1 elisa kit
Reactivity
Human
Specificity
Human Signal transducer and activator of transcription 1 (STAT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAT1. No significant cross-reactivity or interference between Human STAT1 and analogues was observed.
Sequence Length
750
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Signal transducer and activator of transcription 1 (STAT1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Signal transducer and activator of transcription 1 (STAT1) Standard Curve.)
Related Product Information for STAT1 elisa kit
Description: This Human Signal transducer and activator of transcription 1 (STAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT1 in samples. An antibody specific for STAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: STAT1 is a member of the Signal Transducers and Activators of Transcription family of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type I or type II interferons. In response to IFN-gamma; stimulation, STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma Activated Sequence) promoter element; in response to either IFN-alpha or IFN-beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind the ISRE (Interferon Stimulated Response Element) promoter element. In either case, binding of the promoter element leads to an increased expression of ISG (Interferon Stimulated Genes).Expression of STAT1 can be induced with diallyl disulfide, a compound in garlic.
Background: STAT1 is a member of the Signal Transducers and Activators of Transcription family of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type I or type II interferons. In response to IFN-gamma; stimulation, STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma Activated Sequence) promoter element; in response to either IFN-alpha or IFN-beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind the ISRE (Interferon Stimulated Response Element) promoter element. In either case, binding of the promoter element leads to an increased expression of ISG (Interferon Stimulated Genes).Expression of STAT1 can be induced with diallyl disulfide, a compound in garlic.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
signal transducer and activator of transcription 1-alpha/beta isoform alpha
NCBI Official Synonym Full Names
signal transducer and activator of transcription 1
NCBI Official Symbol
STAT1
NCBI Official Synonym Symbols
CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
NCBI Protein Information
signal transducer and activator of transcription 1-alpha/beta
UniProt Protein Name
Signal transducer and activator of transcription 1-alpha/beta
UniProt Gene Name
STAT1
UniProt Entry Name
STAT1_HUMAN
Similar Products
Product Notes
The Human STAT1 stat1 (Catalog #AAA330591) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330591 ELISA Kit recognizes Human STAT1. It is sometimes possible for the material contained within the vial of "Signal Transducer And Activator Of Transcription 1 (STAT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.