Standard Curve (Sample)
(Fig.1. Human Signal transducer and activator of transcription 6 (STAT6) Standard Curve.)
Human Signal Transducer And Activator Of Transcription 6 (STAT6) ELISA Kit | STAT6 elisa kit
Human Signal Transducer And Activator Of Transcription 6 (STAT6) ELISA Kit
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Human
Synonyms
Signal Transducer And Activator Of Transcription 6 (STAT6); N/A; Human Signal Transducer And Activator Of Transcription 6 (STAT6) ELISA Kit; STAT6; D12S1644; IL-4-STAT; STAT6B; STAT6C; STAT; interleukin4-induced; signal transducer and activator of transcription 6; transcription factor IL-4 STAT; STAT6 elisa kit
Reactivity
Human
Specificity
Human Signal transducer and activator of transcription 6 (STAT6) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAT6. No significant cross-reactivity or interference between Human STAT6 and analogues was observed.
Sequence Length
847
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Signal transducer and activator of transcription 6 (STAT6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Signal transducer and activator of transcription 6 (STAT6) Standard Curve.)
Related Product Information for STAT6 elisa kit
Description: This Human Signal transducer and activator of transcription 6 (STAT6) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT6 in samples. An antibody specific for STAT6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STAT6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.
Background: STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
signal transducer and activator of transcription 6 isoform 1
NCBI Official Synonym Full Names
signal transducer and activator of transcription 6
NCBI Official Symbol
STAT6
NCBI Official Synonym Symbols
STAT6B; STAT6C; D12S1644; IL-4-STAT
NCBI Protein Information
signal transducer and activator of transcription 6
UniProt Protein Name
Signal transducer and activator of transcription 6
UniProt Gene Name
STAT6
UniProt Entry Name
STAT6_HUMAN
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Product Notes
The Human STAT6 stat6 (Catalog #AAA330597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330597 ELISA Kit recognizes Human STAT6. It is sometimes possible for the material contained within the vial of "Signal Transducer And Activator Of Transcription 6 (STAT6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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