Standard Curve (Sample)
(Fig.1. Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1) Standard Curve.)
Human Single-Strand Selective Monofunctional Uracil DNA Glycosylase (SMUG1) ELISA Kit | SMUG1 elisa kit
Human Single-Strand Selective Monofunctional Uracil DNA Glycosylase (SMUG1) ELISA Kit
Gene Names
SMUG1; FDG; UNG3; HMUDG
Reactivity
Human
Synonyms
Single-Strand Selective Monofunctional Uracil DNA Glycosylase (SMUG1); N/A; Human Single-Strand Selective Monofunctional Uracil DNA Glycosylase (SMUG1) ELISA Kit; SMUG1; FDG; HMUDG; MGC104370; UNG3; single-strand selective monofunctional uracil DNA glycosylase; SMUG1 elisa kit
Reactivity
Human
Specificity
Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMUG1. No significant cross-reactivity or interference between Human SMUG1 and analogues was observed.
Sequence Length
270
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1) Standard Curve.)
Related Product Information for SMUG1 elisa kit
Description: This Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1) ELISA Kit employs a two-site sandwich ELISA to quantitate SMUG1 in samples. An antibody specific for SMUG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SMUG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMUG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMUG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair. The deduced 270-amino acid protein has a calculated molecular mass of about 30 kD. Human SMUG1 shares 60% identity with the Xenopus protein. Fluorescence-tagged SMUG1 localized predominantly to the nucleus of transiently transfected HeLa cells. SMUG1 showed broader substrate specificity than UNG2, and AP endonuclease had a strong stimulatory effect on SMUG1 against double-stranded uracil, apparently due to enhance dissociation of SMUG1 from AP sites in double-stranded DNA. SMUG1 accumulated within nucleoli in cultured epithelial cells, while UNG2 was excluded from nucleoli.
Background: SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair. The deduced 270-amino acid protein has a calculated molecular mass of about 30 kD. Human SMUG1 shares 60% identity with the Xenopus protein. Fluorescence-tagged SMUG1 localized predominantly to the nucleus of transiently transfected HeLa cells. SMUG1 showed broader substrate specificity than UNG2, and AP endonuclease had a strong stimulatory effect on SMUG1 against double-stranded uracil, apparently due to enhance dissociation of SMUG1 from AP sites in double-stranded DNA. SMUG1 accumulated within nucleoli in cultured epithelial cells, while UNG2 was excluded from nucleoli.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
single-strand selective monofunctional uracil DNA glycosylase isoform 1
NCBI Official Synonym Full Names
single-strand-selective monofunctional uracil-DNA glycosylase 1
NCBI Official Symbol
SMUG1
NCBI Official Synonym Symbols
FDG; UNG3; HMUDG
NCBI Protein Information
single-strand selective monofunctional uracil DNA glycosylase
UniProt Protein Name
Single-strand selective monofunctional uracil DNA glycosylase
UniProt Gene Name
SMUG1
UniProt Entry Name
SMUG1_HUMAN
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Product Notes
The Human SMUG1 smug1 (Catalog #AAA330777) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330777 ELISA Kit recognizes Human SMUG1. It is sometimes possible for the material contained within the vial of "Single-Strand Selective Monofunctional Uracil DNA Glycosylase (SMUG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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