Standard Curve (Sample)
(Fig.1. Human Slit homolog 3 protein (SLIT3) Standard Curve.)
Human Slit Homolog 3 Protein (SLIT3) ELISA Kit | SLIT3 elisa kit
Human Slit Homolog 3 Protein (SLIT3) ELISA Kit
Gene Names
SLIT3; MEGF5; SLIL2; SLIT1; slit2; Slit-3
Reactivity
Human
Synonyms
Slit Homolog 3 Protein (SLIT3); N/A; Human Slit Homolog 3 Protein (SLIT3) ELISA Kit; SLIT3; FLJ10764; MEGF5; SLIL2; SLIT1; Slit-3; slit2; slit homolog 3; SLIT3 elisa kit
Reactivity
Human
Specificity
Human Slit homolog 3 protein (SLIT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLIT3. No significant cross-reactivity or interference between Human SLIT3 and analogues was observed.
Sequence Length
1523
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Slit homolog 3 protein (SLIT3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Slit homolog 3 protein (SLIT3) Standard Curve.)
Related Product Information for SLIT3 elisa kit
Description: This Human Slit homolog 3 protein (SLIT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT3 in samples. An antibody specific for SLIT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLIT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The SLIT3 cDNA encodes a 1,523-amino acid polypeptide with 41.1% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT3 gene was expressed as a major 5.5- and a minor 9.5-kb transcript primarily in the thyroid. SLIT1 and SLIT2 mRNAs were primarily expressed in the brain and spinal cord, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
Background: The SLIT3 cDNA encodes a 1,523-amino acid polypeptide with 41.1% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT3 gene was expressed as a major 5.5- and a minor 9.5-kb transcript primarily in the thyroid. SLIT1 and SLIT2 mRNAs were primarily expressed in the brain and spinal cord, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
slit homolog 3 protein isoform 2
NCBI Official Synonym Full Names
slit guidance ligand 3
NCBI Official Symbol
SLIT3
NCBI Official Synonym Symbols
MEGF5; SLIL2; SLIT1; slit2; Slit-3
NCBI Protein Information
slit homolog 3 protein
UniProt Protein Name
Slit homolog 3 protein
UniProt Gene Name
SLIT3
UniProt Synonym Gene Names
KIAA0814; MEGF5; SLIL2; Slit-3
UniProt Entry Name
SLIT3_HUMAN
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Product Notes
The Human SLIT3 slit3 (Catalog #AAA330791) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330791 ELISA Kit recognizes Human SLIT3. It is sometimes possible for the material contained within the vial of "Slit Homolog 3 Protein (SLIT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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