Standard Curve (Sample)
Human SOD1 ELISA Kit | SOD1 elisa kit
Human SOD1 ELISA Kit
Gene Names
SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
Reactivity
Human
Applications
ELISA
Synonyms
SOD1; N/A; Human SOD1 ELISA Kit; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1; SOD1 elisa kit
Reactivity
Human
Specificity
The Human SOD1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human SOD1 proteins within the range of 78 pg/ml - 5000 pg/ml.
Sequence Length
154
Samples
Serum, plasma
Sensitivity
Typically less than 40 pg/ml.
Applicable Applications for SOD1 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SOD1 elisa kit
Principle of the Assay: The Human SOD1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SOD1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human SOD1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SOD1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SOD1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SOD1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Cu-Zn superoxide dismutase-1 (SOD-1) is a well characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer's disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.
Background/Introduction: Cu-Zn superoxide dismutase-1 (SOD-1) is a well characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer's disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,936 Da
NCBI Official Full Name
Superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 1, soluble
NCBI Official Symbol
SOD1
NCBI Official Synonym Symbols
ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
NCBI Protein Information
superoxide dismutase [Cu-Zn]
UniProt Protein Name
Superoxide dismutase [Cu-Zn]
UniProt Gene Name
SOD1
UniProt Synonym Gene Names
hSod1
UniProt Entry Name
SODC_HUMAN
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Product Notes
The Human SOD1 sod1 (Catalog #AAA261723) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261723 ELISA Kit recognizes Human SOD1. AAA Biotech's SOD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the SOD1 sod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOD1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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