Standard Curve (Sample)
(Fig.1. Human Sodium/iodide cotransporter (SLC5A5) Standard Curve.)
Human Sodium/Iodide Cotransporter (SLC5A5) ELISA Kit | SLC5A5 elisa kit
Human Sodium/Iodide Cotransporter (SLC5A5) ELISA Kit
Gene Names
SLC5A5; NIS; TDH1
Reactivity
Human
Synonyms
Sodium/Iodide Cotransporter (SLC5A5); N/A; Human Sodium/Iodide Cotransporter (SLC5A5) ELISA Kit; SLC5A5; NIS; TDH1; Na(+)/I(-) cotransporter; SLC5A5 elisa kit
Reactivity
Human
Specificity
Human Sodium/iodide cotransporter (SLC5A5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC5A5. No significant cross-reactivity or interference between Human SLC5A5 and analogues was observed.
Sequence Length
643
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Sodium/iodide cotransporter (SLC5A5) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Sodium/iodide cotransporter (SLC5A5) Standard Curve.)
Related Product Information for SLC5A5 elisa kit
Description: This Human Sodium/iodide cotransporter (SLC5A5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC5A5 in samples. An antibody specific for SLC5A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC5A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The sodium-iodide symporter (NIS) is an ion pump that actively transports iodide (I-) across the basolateral membrane into thyroid epithelial cells. The NIS a member of the sodium/solute symporter family.Active transport of iodine into the thyroid gland is an important step in the process of iodide organification and the formation of triiodothyronine (T3) and thyroxine (T4). The symporter is stimulated by TSH, thereby increasing iodine uptake and iodination of tyrosine.The pump symports two sodium ions for every iodide ion. This symporter is driven by low internal sodium concentration in the thyroid epithelial cells, via facilitated diffusion, caused by the sodium/potassium ATPase pump.This pump is the target of certain anion based inhibitors such as perchlorate, pertechnetate, and thiocyanate.
Background: The sodium-iodide symporter (NIS) is an ion pump that actively transports iodide (I-) across the basolateral membrane into thyroid epithelial cells. The NIS a member of the sodium/solute symporter family.Active transport of iodine into the thyroid gland is an important step in the process of iodide organification and the formation of triiodothyronine (T3) and thyroxine (T4). The symporter is stimulated by TSH, thereby increasing iodine uptake and iodination of tyrosine.The pump symports two sodium ions for every iodide ion. This symporter is driven by low internal sodium concentration in the thyroid epithelial cells, via facilitated diffusion, caused by the sodium/potassium ATPase pump.This pump is the target of certain anion based inhibitors such as perchlorate, pertechnetate, and thiocyanate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sodium/iodide cotransporter
NCBI Official Synonym Full Names
solute carrier family 5 member 5
NCBI Official Symbol
SLC5A5
NCBI Official Synonym Symbols
NIS; TDH1
NCBI Protein Information
sodium/iodide cotransporter
UniProt Protein Name
Sodium/iodide cotransporter
UniProt Gene Name
SLC5A5
UniProt Synonym Gene Names
NIS; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter
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Product Notes
The Human SLC5A5 slc5a5 (Catalog #AAA330807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330807 ELISA Kit recognizes Human SLC5A5. It is sometimes possible for the material contained within the vial of "Sodium/Iodide Cotransporter (SLC5A5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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