Human Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit | SLC16A1 elisa kit

Human Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit

Cat. Num. AAA14025

• Gene Names: SLC16A1; MCT; HHF7; MCT1; MCT1D
• Reactivity: Human
• Synonyms: Solute Carrier Family 16, Member 1 (SLC16A1); N/A; Human Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit; MCT1; MCT; Monocarboxylate Transporter 1; Monocarboxylic Acid Transporter 1; SLC16A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLC16A1. No significant cross-reactivity or interference between SLC16A1 and analogues was observed.
• Sequence Length: 500
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLC16A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLC16A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for SLC16A1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLC16A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SLC16A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SLC16A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SLC16A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLC16A1 in human tissue homogenates, cell lysates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 262073007
• NCBI GeneID: 6566
• NCBI Accession #: NP_001159968.1
• NCBI GenBank Nucleotide #: NM_001166496.1
• UniProt Accession #: P53985; Q49A45; Q5T8R6; Q9NSJ9
• Molecular Weight: 46,234 Da
• NCBI Official Full Name: monocarboxylate transporter 1
• NCBI Official Synonym Full Names: solute carrier family 16 member 1
• NCBI Official Symbol: SLC16A1
• NCBI Official Synonym Symbols: MCT; HHF7; MCT1; MCT1D
• NCBI Protein Information: monocarboxylate transporter 1
• UniProt Protein Name: Monocarboxylate transporter 1
• UniProt Gene Name: SLC16A1
• UniProt Synonym Gene Names: MCT1; MCT 1