Standard Curve (Sample)
(Fig.1. Human Sorcin (SRI) Standard Curve.)
Human Sorcin (SRI) ELISA Kit | SRI elisa kit
Human Sorcin (SRI) ELISA Kit
Gene Names
SRI; SCN; V19; CP22; CP-22
Reactivity
Human
Synonyms
Sorcin (SRI); N/A; Human Sorcin (SRI) ELISA Kit; SRI; FLJ26259; SCN; H_RG167B05.1; calcium binding protein amplified in mutlidrug-resistant cells; SRI elisa kit
Reactivity
Human
Specificity
Human Sorcin (SRI) ELISA Kit has high sensitivity and excellent specificity for detection of Human SRI. No significant cross-reactivity or interference between Human SRI and analogues was observed.
Sequence Length
195
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Sorcin (SRI) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Sorcin (SRI) Standard Curve.)
Related Product Information for SRI elisa kit
Description: This Human Sorcin (SRI) ELISA Kit employs a two-site sandwich ELISA to quantitate SRI in samples. An antibody specific for SRI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The 22-kD sorcin protein was overexpressed in HOB1/VCR-1.0 cells, a multidrug-resistant human B immunoblastic lymphoma cell line. By PCR with primers based on the sequence of hamster sorcin, the authors recovered a partial human sorcin cDNA. Using the partial cDNA to screen a HOB1/VCR-1.0 library, they isolated cDNAs corresponding to the entire sorcin coding region. The predicted 198-amino acid human protein shares 95% sequence identity with hamster sorcin. Human sorcin contains 4 EF-hand calcium-binding motifs. Purified protein bound calcium in vitro. Southern and Northern blot analysis revealed that the sorcin gene is amplified and overexpressed in HOB1/VCR-1.0 cells. No expression was detected in parental HOB1 cells.
Background: The 22-kD sorcin protein was overexpressed in HOB1/VCR-1.0 cells, a multidrug-resistant human B immunoblastic lymphoma cell line. By PCR with primers based on the sequence of hamster sorcin, the authors recovered a partial human sorcin cDNA. Using the partial cDNA to screen a HOB1/VCR-1.0 library, they isolated cDNAs corresponding to the entire sorcin coding region. The predicted 198-amino acid human protein shares 95% sequence identity with hamster sorcin. Human sorcin contains 4 EF-hand calcium-binding motifs. Purified protein bound calcium in vitro. Southern and Northern blot analysis revealed that the sorcin gene is amplified and overexpressed in HOB1/VCR-1.0 cells. No expression was detected in parental HOB1 cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sorcin isoform C
NCBI Official Synonym Full Names
sorcin
NCBI Official Symbol
SRI
NCBI Official Synonym Symbols
SCN; V19; CP22; CP-22
NCBI Protein Information
sorcin
UniProt Protein Name
Sorcin
UniProt Gene Name
SRI
UniProt Synonym Gene Names
CP22
UniProt Entry Name
SORCN_HUMAN
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Product Notes
The Human SRI sri (Catalog #AAA330575) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330575 ELISA Kit recognizes Human SRI. It is sometimes possible for the material contained within the vial of "Sorcin (SRI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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