Human Sphingosine 1 Phosphate (Sphingosine 1 Phosphate) ELISA Kit | AT3G58490 elisa kit

Human Sphingosine 1 Phosphate (Sphingosine 1 Phosphate)

Cat. Num. AAA17752

• Reactivity: Human
• Synonyms: Sphingosine 1 Phosphate (Sphingosine 1 Phosphate); N/A; Human Sphingosine 1 Phosphate (Sphingosine 1 Phosphate); AT3G58490 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S1P. No significant cross-reactivity or interference between S1P and analogues was observed.
• Sequence Length: 416
• Assay Type: Competitive
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 3.125-200ng/ml
• Sensitivity: 1.875ng/ml

• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for AT3G58490 elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15231046
• NCBI GeneID: 825018
• NCBI Accession #: NP_191408.1
• NCBI GenBank Nucleotide #: NM_115711.3
• Molecular Weight: 46,169 Da
• NCBI Official Full Name: sphingoid phosphate phosphatase 1
• NCBI Official Symbol: AT3G58490
• NCBI Protein Information: sphingoid phosphate phosphatase 1
• UniProt Protein Name: Lipid phosphate phosphatase delta
• UniProt Gene Name: LPPD
• UniProt Synonym Gene Names: SPP1; AtLPPD; AtSSP1; AtSPPASE
• UniProt Entry Name: LPPD_ARATH