Standard Curve (Sample)
(Fig.1. Human Spleen tyrosine kinase (SYK) Standard Curve.)
Human Spleen Tyrosine Kinase (SYK) ELISA Kit | SYK elisa kit
Human Spleen Tyrosine Kinase (SYK) ELISA Kit
Gene Names
SYK; p72-Syk
Reactivity
Human
Synonyms
Spleen Tyrosine Kinase (SYK); N/A; Human Spleen Tyrosine Kinase (SYK) ELISA Kit; SYK; DKFZp313N1010; FLJ25043; FLJ37489; OTTHUMP00000021628; OTTHUMP00000021629; SYK elisa kit
Reactivity
Human
Specificity
Human Spleen tyrosine kinase (SYK) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYK. No significant cross-reactivity or interference between Human SYK and analogues was observed.
Sequence Length
612
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Spleen tyrosine kinase (SYK) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Spleen tyrosine kinase (SYK) Standard Curve.)
Related Product Information for SYK elisa kit
Description: This Human Spleen tyrosine kinase (SYK) ELISA Kit employs a two-site sandwich ELISA to quantitate SYK in samples. An antibody specific for SYK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SYKis a member of the Syk family of tyrosine kinases. While Syk and Zap-70 are primarily expressed in hematopoietic tissues, there is expression of Syk in a variety of tissues. Within B and T cells respectively, Syk and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. Syk plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. The role of Syk in epithelial malignancies is controversial. Several authors have suggested that abnormal Syk function facilitates transformation in Nasopharyngeal carcinoma and head and neck cancer while other authors have suggested a tumor suppressor role in breast and gastric cancer.
Background: SYKis a member of the Syk family of tyrosine kinases. While Syk and Zap-70 are primarily expressed in hematopoietic tissues, there is expression of Syk in a variety of tissues. Within B and T cells respectively, Syk and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. Syk plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. The role of Syk in epithelial malignancies is controversial. Several authors have suggested that abnormal Syk function facilitates transformation in Nasopharyngeal carcinoma and head and neck cancer while other authors have suggested a tumor suppressor role in breast and gastric cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tyrosine-protein kinase SYK isoform Syk(S)
NCBI Official Synonym Full Names
spleen associated tyrosine kinase
NCBI Official Symbol
SYK
NCBI Official Synonym Symbols
p72-Syk
NCBI Protein Information
tyrosine-protein kinase SYK
UniProt Protein Name
Tyrosine-protein kinase SYK
UniProt Gene Name
SYK
UniProt Entry Name
KSYK_HUMAN
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Product Notes
The Human SYK syk (Catalog #AAA330625) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330625 ELISA Kit recognizes Human SYK. It is sometimes possible for the material contained within the vial of "Spleen Tyrosine Kinase (SYK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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