Standard Curve (Sample)
(Fig.1. Human SPOC domain-containing protein 1 (SPOCD1) Standard Curve.)
Human SPOC Domain-Containing Protein 1 (SPOCD1) ELISA Kit | SPOCD1 elisa kit
Human SPOC Domain-Containing Protein 1 (SPOCD1) ELISA Kit
Gene Names
SPOCD1; PPP1R146
Reactivity
Human
Synonyms
SPOC Domain-Containing Protein 1 (SPOCD1); N/A; Human SPOC Domain-Containing Protein 1 (SPOCD1) ELISA Kit; SPOCD1; FLJ25348; FLJ39908; RP11-84A19.1; SPOCD1 elisa kit
Reactivity
Human
Specificity
Human SPOC domain-containing protein 1 (SPOCD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPOCD1. No significant cross-reactivity or interference between Human SPOCD1 and analogues was observed.
Sequence Length
1203
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human SPOC domain-containing protein 1 (SPOCD1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human SPOC domain-containing protein 1 (SPOCD1) Standard Curve.)
Related Product Information for SPOCD1 elisa kit
Description: This Human SPOC domain-containing protein 1 (SPOCD1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCD1 in samples. An antibody specific for SPOCD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPOCD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPOCD1 Contains 1 SPOC domain. Contains 1 TFIIS central domain.The Split ends (Spen) family are large proteins characterised by N-terminal RNA recognition motifs (RRMs) and a conserved SPOC (Spen paralog and ortholog C-terminal) domain.The widely distributed DIO (Death inducer-obliterator) family is related to cancer and apoptosis and offers new clues about SPOC domain functionality. TFIIS is a transcription elongation factor, which directly binds the transcription motor, RNA Polymerase II and allows it to read through various transcription arrest sites. TFIIS, a transcription elongation factor, and its affect on proliferation of cancer cells in culture. Knockdown of TFIIS reduced cancer cell proliferation in breast, lung and pancreatic cancer cell lines.
Background: SPOCD1 Contains 1 SPOC domain. Contains 1 TFIIS central domain.The Split ends (Spen) family are large proteins characterised by N-terminal RNA recognition motifs (RRMs) and a conserved SPOC (Spen paralog and ortholog C-terminal) domain.The widely distributed DIO (Death inducer-obliterator) family is related to cancer and apoptosis and offers new clues about SPOC domain functionality. TFIIS is a transcription elongation factor, which directly binds the transcription motor, RNA Polymerase II and allows it to read through various transcription arrest sites. TFIIS, a transcription elongation factor, and its affect on proliferation of cancer cells in culture. Knockdown of TFIIS reduced cancer cell proliferation in breast, lung and pancreatic cancer cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
SPOC domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
SPOC domain containing 1
NCBI Official Symbol
SPOCD1
NCBI Official Synonym Symbols
PPP1R146
NCBI Protein Information
SPOC domain-containing protein 1
UniProt Protein Name
SPOC domain-containing protein 1
UniProt Gene Name
SPOCD1
UniProt Entry Name
SPOC1_HUMAN
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Product Notes
The Human SPOCD1 spocd1 (Catalog #AAA330551) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330551 ELISA Kit recognizes Human SPOCD1. It is sometimes possible for the material contained within the vial of "SPOC Domain-Containing Protein 1 (SPOCD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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