Standard Curve (Sample)
Human Sulfatase-Modifying Factor 1 (SUMF1) ELISA Kit | SUMF1 elisa kit
Human Sulfatase-Modifying Factor 1 (SUMF1) ELISA Kit
Gene Names
SUMF1; FGE; UNQ3037; AAPA3037
Reactivity
Human
Synonyms
Sulfatase-Modifying Factor 1 (SUMF1); N/A; Human Sulfatase-Modifying Factor 1 (SUMF1) ELISA Kit; SUMF1; UNQ3037; AAPA3037; FGE; MGC131853; MGC150436; C-alpha-formylglycine-generating enzyme; FGly-generating enzyme; SUMF1 elisa kit
Reactivity
Human
Specificity
Human Sulfatase-modifying factor 1 (SUMF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SUMF1. No significant cross-reactivity or interference between Human SUMF1 and analogues was observed.
Sequence Length
349
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
15 pg/ml - 240 pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SUMF1 elisa kit
Background: Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER). The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD), a lysosomal storage disorder (Roeser et al., 2006).Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart.
Principle of the Assay: Human Sulfatase-modifying factor 1 (SUMF1) ELISA Kit employs a two-site sandwich ELISA to quantitate SUMF1 in samples. An antibody specific for SUMF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SUMF1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SUMF1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SUMF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Sulfatase-modifying factor 1 (SUMF1) ELISA Kit employs a two-site sandwich ELISA to quantitate SUMF1 in samples. An antibody specific for SUMF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SUMF1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SUMF1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SUMF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
formylglycine-generating enzyme isoform 2
NCBI Official Synonym Full Names
sulfatase modifying factor 1
NCBI Official Symbol
SUMF1
NCBI Official Synonym Symbols
FGE; UNQ3037; AAPA3037
NCBI Protein Information
formylglycine-generating enzyme; sulfatase-modifying factor 1
UniProt Protein Name
Sulfatase-modifying factor 1
UniProt Gene Name
SUMF1
UniProt Synonym Gene Names
FGE
UniProt Entry Name
SUMF1_HUMAN
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Product Notes
The Human SUMF1 sumf1 (Catalog #AAA330618) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330618 ELISA Kit recognizes Human SUMF1. It is sometimes possible for the material contained within the vial of "Sulfatase-Modifying Factor 1 (SUMF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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