Human SUMO conjugating enzyme UBC9 (UBE2I) ELISA Kit | UBE2I elisa kit

Human SUMO conjugating enzyme UBC9 (UBE2I) ELISA Kit

Cat. Num. AAA247078

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Reactivity: Human
• Synonyms: SUMO conjugating enzyme UBC9 (UBE2I); N/A; Human SUMO conjugating enzyme UBC9 (UBE2I) ELISA Kit; UBE2I elisa kit

• Reactivity: Human
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL

• Intended Uses: This UBE2I ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human UBE2I. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for UBE2I elisa kit

Principle of the assay: UBE2I ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UBE2I antibody and an UBE2I-HRP conjugate. The assay sample and buffer are incubated together with UBE2I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UBE2I concentration since UBE2I from samples and UBE2I-HRP conjugate compete for the anti-UBE2I antibody binding site. Since the number of sites is limited, as more sites are occupied by UBE2I from the sample, fewer sites are left to bind UBE2I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UBE2I concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 35494003
• NCBI GeneID: 7329
• NCBI Accession #: NP_919237.1
• NCBI GenBank Nucleotide #: NM_194261.2
• Molecular Weight: 18,007 Da
• NCBI Official Full Name: SUMO-conjugating enzyme UBC9
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9
• UniProt Entry Name: UBC9_HUMAN