Human Synaptosomal-associated protein 25 (SNAP25) ELISA Kit | SNAP25 elisa kit
Human Synaptosomal-associated protein 25 (SNAP25) ELISA Kit
Gene Names
Snap25; SNAP-25B; SNAP-25a
Reactivity
Human
Synonyms
Synaptosomal-associated protein 25 (SNAP25); N/A; Human Synaptosomal-associated protein 25 (SNAP25) ELISA Kit; SNAP25 elisa kit
Reactivity
Human
Sequence Length
206
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 20ng/ml
Sensitivity
0.023ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for SNAP25 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Synaptosomal-Associated Protein 25 (also known as SNAP25) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SNAP25 antibody. SNAP25 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SNAP25 Antibody is added and binds to SNAP25 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SNAP25 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SNAP25. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SNAP25 antibody. SNAP25 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SNAP25 Antibody is added and binds to SNAP25 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SNAP25 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SNAP25. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,336 Da
NCBI Official Full Name
synaptosomal-associated protein 25 isoform a
NCBI Official Synonym Full Names
synaptosome associated protein 25
NCBI Official Symbol
Snap25
NCBI Official Synonym Symbols
SNAP-25B; SNAP-25a
NCBI Protein Information
synaptosomal-associated protein 25
UniProt Protein Name
Synaptosomal-associated protein 25
UniProt Gene Name
Snap25
UniProt Synonym Gene Names
Snap; SNAP-25; SUP