Human Syntaxin binding protein 1 (STXBP1) ELISA Kit | STXBP1 elisa kit

Human Syntaxin binding protein 1 (STXBP1) ELISA Kit

Cat. Num. AAA27333

• Gene Names: STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• Reactivity: Human
• Synonyms: Syntaxin binding protein 1 (STXBP1); N/A; Human Syntaxin binding protein 1 (STXBP1) ELISA Kit; STXBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of STXBP1. No significant cross-reactivity or interference between STXBP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STXBP1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for STXBP1 elisa kit

Principle of the Assay: STXBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-STXBP1 antibody and an STXBP1-HRP conjugate. The assay sample and buffer are incubated together with STXBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STXBP1 concentration since STXBP1 from samples and STXBP1-HRP conjugate compete for the anti-STXBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by STXBP1 from the sample, fewer sites are left to bind STXBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STXBP1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This STXBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STXBP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for STXBP1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 16041740
• NCBI GeneID: 6812
• Molecular Weight: 68,736 Da
• NCBI Official Full Name: Syntaxin binding protein 1
• NCBI Official Synonym Full Names: syntaxin binding protein 1
• NCBI Official Symbol: STXBP1
• NCBI Official Synonym Symbols: P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• NCBI Protein Information: syntaxin-binding protein 1; N-Sec1; unc-18A; unc18-1; neuronal SEC1; protein unc-18 homolog 1; protein unc-18 homolog A
• UniProt Protein Name: Syntaxin-binding protein 1
• UniProt Gene Name: STXBP1
• UniProt Synonym Gene Names: UNC18A; Unc18-1; Unc-18A
• UniProt Entry Name: STXB1_HUMAN