Standard Curve (Sample)
(Fig.1. Human T-H glycoprotein (THP) Standard Curve.)
Human T-H Glycoprotein (THP) ELISA Kit | THP elisa kit
Human T-H Glycoprotein (THP) ELISA Kit
Gene Names
UMOD; THP; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADMCKD2
Reactivity
Human
Synonyms
T-H Glycoprotein (THP); N/A; Human T-H Glycoprotein (THP) ELISA Kit; UMOD; ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; THGP; THP; Tamm-Horsfall glycoprotein; uromodulin (uromucoid; Tamm-Horsfall glycoprotein); uromucoid; THP elisa kit
Reactivity
Human
Specificity
Human T-H glycoprotein (THP) ELISA Kit has high sensitivity and excellent specificity for detection of Human UMOD. No significant cross-reactivity or interference between Human UMOD and analogues was observed.
Sequence Length
640
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human T-H glycoprotein (THP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human T-H glycoprotein (THP) Standard Curve.)
Related Product Information for THP elisa kit
Description: This Human T-H glycoprotein (THP) ELISA Kit employs a two-site sandwich ELISA to quantitate UMOD in samples. An antibody specific for UMOD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UMOD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UMOD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UMOD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 and familial juvenile hyperuricemic nephropathy. These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date.
Background: Uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 and familial juvenile hyperuricemic nephropathy. These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
uromodulin isoform a preproprotein
NCBI Official Synonym Full Names
uromodulin
NCBI Official Symbol
UMOD
NCBI Official Synonym Symbols
THP; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADMCKD2
NCBI Protein Information
uromodulin
UniProt Protein Name
Uromodulin
UniProt Gene Name
UMOD
UniProt Synonym Gene Names
THP
UniProt Entry Name
UROM_HUMAN
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Product Notes
The Human THP umod (Catalog #AAA330313) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330313 ELISA Kit recognizes Human THP. It is sometimes possible for the material contained within the vial of "T-H Glycoprotein (THP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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