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product-image-AAA330413_SC15.jpg Standard Curve (Sample)

Human Tenomodulin (TNMD) ELISA Kit | TNMD elisa kit

Human Tenomodulin (TNMD) ELISA Kit

Gene Names
TNMD; TEM; CHM1L; BRICD4
Reactivity
Human
Synonyms
Tenomodulin (TNMD); N/A; Human Tenomodulin (TNMD) ELISA Kit; TNMD; BRICD4; CHM1L; TEM; BRICHOS domain containing 4; CHM1-like; chondromodulin I-like; chondromodulin-IB; myodulin; tendin; TNMD elisa kit
Ordering
Reactivity
Human
Specificity
Human Tenomodulin (TNMD) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNMD. No significant cross-reactivity or interference between Human TNMD and analogues was observed.
Sequence Length
317
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
50 pg/ml - 800 pg/ml
Sensitivity
< 5pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.

Standard Curve (Sample)

product-image-AAA330413_SC15.jpg Standard Curve (Sample)
Related Product Information for TNMD elisa kit
Background: By searching an EST database for sequences similar to mouse Tem, followed by RACE of a human fetus cDNA library, Shukunami et al. (2001) cloned TEM. The deduced 317-amino acid protein contains an N-terminal transmembrane domain and a putative antiangiogenic domain with 8 cysteines. TEM shares 96% amino acid identity with mouse Tem, and it shares 65% identity in a 65-amino acid C-terminal stretch with chondromodulin I (CHM1). Northern blot analysis of several mouse tissues detected a 1.4-kb transcript expressed specifically in skeletal muscle. In situ hybridization revealed mouse Tem expression in dense connective tissue, such as epimysium and tendon.

Principle of the Assay: Human Tenomodulin (TNMD) ELISA Kit employs a two-site sandwich ELISA to quantitate TNMD in samples. An antibody specific for TNMD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNMD present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human TNMD detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TNMD bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tenomodulin
NCBI Official Synonym Full Names
tenomodulin
NCBI Official Symbol
TNMD
NCBI Official Synonym Symbols
TEM; CHM1L; BRICD4
NCBI Protein Information
tenomodulin
UniProt Protein Name
Tenomodulin
UniProt Gene Name
TNMD
UniProt Synonym Gene Names
CHM1L; TeM; hTeM; ChM1L; hChM1L

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Product Notes

The Human TNMD tnmd (Catalog #AAA330413) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330413 ELISA Kit recognizes Human TNMD. It is sometimes possible for the material contained within the vial of "Tenomodulin (TNMD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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