Standard Curve (Sample)
Human Testican-3 (SPOCK3) ELISA Kit | SPOCK3 elisa kit
Human Testican-3 (SPOCK3) ELISA Kit
Gene Names
SPOCK3; TES-3; TICN3; HSAJ1454
Reactivity
Human
Synonyms
Testican-3 (SPOCK3); N/A; Human Testican-3 (SPOCK3) ELISA Kit; SPOCK3; HSAJ1454; TES-3; testican 3; SPOCK3 elisa kit
Reactivity
Human
Sequence Length
433
Assay Type
Sandwich
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPOCK3 elisa kit
Principle of the Assay: Human Testican-3 (SPOCK3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK3 in samples. An antibody specific for SPOCK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPOCK3 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SPOCK3 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SPOCK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Testican 3, encoded by the SPOCK3 gene, is a proteoglycan expressed in brain (1). Human and mouse Testican 3 share 90% amino acid sequence identity, indicating a conserved function (2, 3). Testican 3 contains Ca2+-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to Testicans, is responsible for the inhibition of Testican 3 towards MMP-14/MT1-MMP activation of MMP-2 (1).
Background/Introduction: Testican 3, encoded by the SPOCK3 gene, is a proteoglycan expressed in brain (1). Human and mouse Testican 3 share 90% amino acid sequence identity, indicating a conserved function (2, 3). Testican 3 contains Ca2+-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to Testicans, is responsible for the inhibition of Testican 3 towards MMP-14/MT1-MMP activation of MMP-2 (1).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
testican-3 isoform 1
NCBI Official Synonym Full Names
SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3
NCBI Official Symbol
SPOCK3
NCBI Official Synonym Symbols
TES-3; TICN3; HSAJ1454
NCBI Protein Information
testican-3
UniProt Protein Name
Testican-3
UniProt Gene Name
SPOCK3
UniProt Synonym Gene Names
TICN3; UNQ409/PRO771
UniProt Entry Name
TICN3_HUMAN
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Product Notes
The Human SPOCK3 spock3 (Catalog #AAA330554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330554 ELISA Kit recognizes Human SPOCK3. It is sometimes possible for the material contained within the vial of "Testican-3 (SPOCK3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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