Standard Curve (Sample)
Human TFEB (Transcription factor EB) ELISA Kit | TFEB elisa kit
Human TFEB (Transcription factor EB) ELISA Kit
Gene Names
TFEB; TCFEB; BHLHE35; ALPHATFEB
Reactivity
Human
Synonyms
TFEB (Transcription factor EB); N/A; Human TFEB (Transcription factor EB) ELISA Kit; Transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35; TFEB; BHLHE35; TFEB elisa kit
Reactivity
Human
Specificity
Specifically recognize TFEB, no obvious cross reaction with other analogues
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TFEB elisa kit
Background: The transcription factor EB (TFEB) plays a pivotal role in the regulation of basic cellular processes, such as lysosomal biogenesis and autophagy. Phosphorylated TFEB is retained in the cytoplasm, whereas dephosphorylated TFEB translocates to the nucleus to induce the transcription of target genes. TFEB is also involved in physiological processes, such as lipid catabolism.Diseases associated with TFEB include Renal Cell Carcinoma, Xp11-Associated and Pycnodysostosis.Among its related pathways are Autophagy in pancreatic ductal adenocarcinoma and Type 2 papillary renal cell carcinoma.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TFEB antibody was precoated onto the 96-well plate. The biotin conjugated anti TFEB antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TFEB conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TFEB in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TFEB antibody was precoated onto the 96-well plate. The biotin conjugated anti TFEB antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TFEB conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TFEB in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52 KD
NCBI Official Full Name
transcription factor EB isoform 2
NCBI Official Synonym Full Names
transcription factor EB
NCBI Official Symbol
TFEB
NCBI Official Synonym Symbols
TCFEB; BHLHE35; ALPHATFEB
NCBI Protein Information
transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
UniProt Protein Name
Transcription factor EB
UniProt Gene Name
TFEB
UniProt Synonym Gene Names
BHLHE35; bHLHe35
UniProt Entry Name
TFEB_HUMAN
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Product Notes
The Human TFEB tfeb (Catalog #AAA27645) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27645 ELISA Kit recognizes Human TFEB. It is sometimes possible for the material contained within the vial of "TFEB (Transcription factor EB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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