Standard Curve (Sample)
Human TGFBI/beta IG-H3 ELISA Kit | TGFBI elisa kit
Human TGFBI/beta IG-H3 PicoKine ELISA Kit
Gene Names
TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
Reactivity
Human
Synonyms
TGFBI/beta IG-H3; N/A; Human TGFBI/beta IG-H3 PicoKine ELISA Kit; Transforming growth factor-beta-induced protein ig-h3; transforming growth factor, beta-induced, 68kDa; Beta ig-h3; beta-IG-H3; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBI elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TGFBI
Sequence Length
683
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TGFBI elisa kit
Background: Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Principle of the Assay: The Picokine™ Human TGFBI Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TGFBI with a 96-well strip plate that is pre-coated with antibody specific for TGFBI. The detection antibody is a biotinylated antibody specific for TGFBI. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TGFBI with immunogen: Expression system for standard: NS0; Immunogen sequence: G24-H683. The kit is analytically validated with ready-to-use reagents. To measure Human TGFBI, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TGFBI in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TGFBI in the sample.
Principle of the Assay: The Picokine™ Human TGFBI Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TGFBI with a 96-well strip plate that is pre-coated with antibody specific for TGFBI. The detection antibody is a biotinylated antibody specific for TGFBI. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TGFBI with immunogen: Expression system for standard: NS0; Immunogen sequence: G24-H683. The kit is analytically validated with ready-to-use reagents. To measure Human TGFBI, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TGFBI in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TGFBI in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
74,681 Da
NCBI Official Full Name
Transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor, beta-induced, 68kDa
NCBI Official Symbol
TGFBI
NCBI Official Synonym Symbols
CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3; RGD-CAP; beta ig-h3; kerato-epithelin; RGD-containing collagen-associated protein; transforming growth factor, beta-induced, 68kD
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
UniProt Gene Name
TGFBI
UniProt Synonym Gene Names
BIGH3; Beta ig-h3; RGD-CAP
UniProt Entry Name
BGH3_HUMAN
Similar Products
Product Notes
The Human TGFBI tgfbi (Catalog #AAA11621) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11621 ELISA Kit recognizes Human TGFBI. It is sometimes possible for the material contained within the vial of "TGFBI/beta IG-H3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
+1 (619) 604-9440