Standard Curve (Sample)
Human TGFBR3/Tgf Beta Riii ELISA Kit | TGFBR3 elisa kit
Human TGFBR3/Tgf Beta Riii PicoKine Fast ELISA Kit
Gene Names
TGFBR3; BGCAN; betaglycan
Reactivity
Human
Synonyms
TGFBR3/Tgf Beta Riii; N/A; Human TGFBR3/Tgf Beta Riii PicoKine Fast ELISA Kit; human TGFBR3; Transforming growth factor beta receptor type 3; TGF-beta receptor type 3; TGFR-3; Betaglycan; Transforming growth factor beta receptor III; TGF-beta receptor type III; TGFBR3; TGFBR3 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TGFBR3
Sequence Length
850
Samples
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TGFBR3 elisa kit
Principle of the Assay: The Picokine™ Human TGFBR3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TGFBR3 with a 96-well strip plate that is pre-coated with antibody specific for TGFBR3. The detection antibody is a biotinylated antibody specific for TGFBR3. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TGFBR3 with immunogen: NSO, G21-D781. The kit is analytically validated with ready to use reagents. To measure Human TGFBR3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human TGFBR3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TGFBR3 in the sample.!!Background/Introduction: Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell- surfacechondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, nonredundant role in TGF-beta signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transforming growth factor beta receptor type 3 isoform b
NCBI Official Synonym Full Names
transforming growth factor beta receptor 3
NCBI Official Symbol
TGFBR3
NCBI Official Synonym Symbols
BGCAN; betaglycan
NCBI Protein Information
transforming growth factor beta receptor type 3
UniProt Protein Name
Transforming growth factor beta receptor type 3
UniProt Gene Name
TGFBR3
UniProt Synonym Gene Names
TGF-beta receptor type 3; TGFR-3
UniProt Entry Name
TGBR3_HUMAN
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Product Notes
The Human TGFBR3 tgfbr3 (Catalog #AAA125371) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125371 ELISA Kit recognizes Human TGFBR3. It is sometimes possible for the material contained within the vial of "TGFBR3/Tgf Beta Riii, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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