Standard Curve (Sample)
(Fig.1. Human Thioredoxin reductase 2, mitochondrial (TXNRD2) Standard Curve.)
Human Thioredoxin Reductase 2, Mitochondrial (TXNRD2) ELISA Kit | TXNRD2 elisa kit
Human Thioredoxin Reductase 2, Mitochondrial (TXNRD2) ELISA Kit
Gene Names
TXNRD2; TR; TR3; SELZ; GCCD5; TRXR2; TR-BETA
Reactivity
Human
Synonyms
Thioredoxin Reductase 2, Mitochondrial (TXNRD2); N/A; Human Thioredoxin Reductase 2, Mitochondrial (TXNRD2) ELISA Kit; TXNRD2; SELZ; TR; TR-BETA; TR3; TRXR2; OTTHUMP00000195708; selenoprotein Z; thioredoxin reductase 3; thioredoxin reductase beta; TXNRD2 elisa kit
Reactivity
Human
Specificity
Human Thioredoxin reductase 2, mitochondrial (TXNRD2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TXNRD2. No significant cross-reactivity or interference between Human TXNRD2 and analogues was observed.
Sequence Length
524
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Thioredoxin reductase 2, mitochondrial (TXNRD2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Thioredoxin reductase 2, mitochondrial (TXNRD2) Standard Curve.)
Related Product Information for TXNRD2 elisa kit
Description: This Human Thioredoxin reductase 2, mitochondrial (TXNRD2) ELISA Kit employs a two-site sandwich ELISA to quantitate TXNRD2 in samples. An antibody specific for TXNRD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXNRD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXNRD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXNRD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thioredoxin reductase (TR) is a dimeric NADPH-dependent FAD containing enzyme that catalyzes the reduction of the active site disulfide of thioredoxin and other substrates. TR is a member of a family of pyridine nucleotide-disulfide oxidoreductases and is a key enzyme in the regulation of the intracellular redox environment. Three thioredoxin reductase genes have been found that encode selenocysteine containing proteins. TrxR2 partially overlaps the COMT gene on chromosome 22.Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes.
Background: Thioredoxin reductase (TR) is a dimeric NADPH-dependent FAD containing enzyme that catalyzes the reduction of the active site disulfide of thioredoxin and other substrates. TR is a member of a family of pyridine nucleotide-disulfide oxidoreductases and is a key enzyme in the regulation of the intracellular redox environment. Three thioredoxin reductase genes have been found that encode selenocysteine containing proteins. TrxR2 partially overlaps the COMT gene on chromosome 22.Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thioredoxin reductase 2, mitochondrial isoform 1
NCBI Official Synonym Full Names
thioredoxin reductase 2
NCBI Official Symbol
TXNRD2
NCBI Official Synonym Symbols
TR; TR3; SELZ; GCCD5; TRXR2; TR-BETA
NCBI Protein Information
thioredoxin reductase 2, mitochondrial
UniProt Protein Name
Thioredoxin reductase 2, mitochondrial
UniProt Gene Name
TXNRD2
UniProt Synonym Gene Names
KIAA1652; TRXR2; SelZ
UniProt Entry Name
TRXR2_HUMAN
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Product Notes
The Human TXNRD2 txnrd2 (Catalog #AAA330335) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330335 ELISA Kit recognizes Human TXNRD2. It is sometimes possible for the material contained within the vial of "Thioredoxin Reductase 2, Mitochondrial (TXNRD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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