Standard Curve (Sample)
Human TNFRSF14/HVEM ELISA Kit | TNFRSF14/HVEM elisa kit
Human TNFRSF14/HVEM PicoKine Fast ELISA Kit
Gene Names
TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
Reactivity
Human
Synonyms
TNFRSF14/HVEM; N/A; Human TNFRSF14/HVEM PicoKine Fast ELISA Kit; human TNFRSF14; Tumor necrosis factor receptor superfamily member 14; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; Tumor necrosis factor receptor-like 2; TR2; CD270; TNFRSF14; HVEA, HVEM; UNQ329/PRO509; Protein Tnfrsf14; TNFRSF14/HVEM elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TNFRSF14
Sequence Length
283
Samples
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNFRSF14/HVEM elisa kit
Principle of the Assay: The Picokine™ Human TNFRSF14 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNFRSF14 with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF14. The detection antibody is a biotinylated antibody specific for TNFRSF14. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFRSF14 with immunogen: Expression system for standard: NSO; Immunogen sequence: L39-V202. The kit is analytically validated with ready to use reagents. To measure Human TNFRSF14, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human TNFRSF14 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF14 in the sample.!!Background/Introduction: Tumor necrosis factor receptor superfamily member 14(TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 14
NCBI Official Synonym Full Names
TNF receptor superfamily member 14
NCBI Official Symbol
TNFRSF14
NCBI Official Synonym Symbols
TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 14
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 14
UniProt Gene Name
TNFRSF14
UniProt Synonym Gene Names
HVEA; HVEM; Herpesvirus entry mediator A; HveA; TR2
UniProt Entry Name
TNR14_HUMAN
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Product Notes
The Human TNFRSF14/HVEM tnfrsf14 (Catalog #AAA125362) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125362 ELISA Kit recognizes Human TNFRSF14/HVEM. It is sometimes possible for the material contained within the vial of "TNFRSF14/HVEM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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