Standard Curve (Sample)
(Fig.1. Human Transcription termination factor 1 (TTF1) Standard Curve.)
Human Transcription Termination Factor 1 (TTF1) ELISA Kit | TTF1 elisa kit
Human Transcription Termination Factor 1 (TTF1) ELISA Kit
Gene Names
TTF1; TTF-1; TTF-I
Reactivity
Human
Synonyms
Transcription Termination Factor 1 (TTF1); N/A; Human Transcription Termination Factor 1 (TTF1) ELISA Kit; TTF1 elisa kit
Reactivity
Human
Specificity
Human Transcription termination factor 1 (TTF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TTF1. No significant cross-reactivity or interference between Human TTF1 and analogues was observed.
Sequence Length
390
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Transcription termination factor 1 (TTF1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Transcription termination factor 1 (TTF1) Standard Curve.)
Related Product Information for TTF1 elisa kit
Description: This Human Transcription termination factor 1 (TTF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TTF1 in samples. An antibody specific for TTF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TTF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TTF2 has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.The deduced 990-amino acid TTF2 protein shares 43% amino acid sequence identity with Drosophila factor 2. These proteins contain 7 motifs found in helicase superfamilies 1 and 2; 7 additional motifs shared only by SWI2/SNF2 family members; and 3 conserved motifs and a nuclear localization signal that are not conserved among the SWI2/SNF2 family. Similar to Drosophila factor 2, recombinant human TTF2 displayed a strong double-stranded DNA-dependent ATPase activity that was inhibited by single-stranded DNA and exhibited RNA polymerase II termination activity.
Background: TTF2 has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.The deduced 990-amino acid TTF2 protein shares 43% amino acid sequence identity with Drosophila factor 2. These proteins contain 7 motifs found in helicase superfamilies 1 and 2; 7 additional motifs shared only by SWI2/SNF2 family members; and 3 conserved motifs and a nuclear localization signal that are not conserved among the SWI2/SNF2 family. Similar to Drosophila factor 2, recombinant human TTF2 displayed a strong double-stranded DNA-dependent ATPase activity that was inhibited by single-stranded DNA and exhibited RNA polymerase II termination activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transcription termination factor 1 isoform 2
NCBI Official Synonym Full Names
transcription termination factor 1
NCBI Official Symbol
TTF1
NCBI Official Synonym Symbols
TTF-1; TTF-I
NCBI Protein Information
transcription termination factor 1
UniProt Protein Name
Transcription termination factor 1
UniProt Gene Name
TTF1
UniProt Synonym Gene Names
TTF-1; TTF-I
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Product Notes
The Human TTF1 ttf1 (Catalog #AAA330343) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330343 ELISA Kit recognizes Human TTF1. It is sometimes possible for the material contained within the vial of "Transcription Termination Factor 1 (TTF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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