Human Transmembrane protein 175 (TMEM175) ELISA Kit | TMEM175 elisa kit

Human Transmembrane protein 175 (TMEM175) ELISA Kit

Cat. Num. AAA246259

• Reactivity: Human
• Synonyms: Transmembrane protein 175 (TMEM175); N/A; Human Transmembrane protein 175 (TMEM175) ELISA Kit; TMEM175 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TMEM175. No significant cross-reactivity or interference between TMEM175 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TMEM175 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for TMEM175 elisa kit

Principle of the Assay: TMEM175 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TMEM175 antibody and an TMEM175-HRP conjugate. The assay sample and buffer are incubated together with TMEM175-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM175 concentration since TMEM175 from samples and TMEM175-HRP conjugate compete for the anti-TMEM175 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM175 from the sample, fewer sites are left to bind TMEM175-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM175 concentration in each sample is interpolated from this standard curve.

Intended Uses: This TMEM175 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TMEM175. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 13477363
• NCBI GeneID: 84286
• Molecular Weight: 42,847 Da
• NCBI Official Full Name: Transmembrane protein 175
• NCBI Official Synonym Full Names: transmembrane protein 175
• NCBI Official Symbol: TMEM175
• NCBI Protein Information: transmembrane protein 175
• UniProt Protein Name: Transmembrane protein 175
• UniProt Gene Name: TMEM175
• UniProt Entry Name: TM175_HUMAN