Standard Curve (Sample)
(Fig.1. Human Transmembrane protein 55A (TMEM55A) Standard Curve.)
Human Transmembrane Protein 55A (TMEM55A) ELISA Kit | TMEM55A elisa kit
Human Transmembrane Protein 55A (TMEM55A) ELISA Kit
Gene Names
PIP4P2; TMEM55A
Reactivity
Human
Synonyms
Transmembrane Protein 55A (TMEM55A); N/A; Human Transmembrane Protein 55A (TMEM55A) ELISA Kit; TMEM55A; DKFZp762O076; PtdIns-4; 5-P(2) 4-phosphatase type; TMEM55A elisa kit
Reactivity
Human
Specificity
Human Transmembrane protein 55A (TMEM55A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM55A. No significant cross-reactivity or interference between Human TMEM55A and analogues was observed.
Sequence Length
257
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Transmembrane protein 55A (TMEM55A) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Transmembrane protein 55A (TMEM55A) Standard Curve.)
Related Product Information for TMEM55A elisa kit
Description: This Human Transmembrane protein 55A (TMEM55A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM55A in samples. An antibody specific for TMEM55A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM55A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM55A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM55A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMEM55A catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate.The deduced 300-amino acid protein contains a central Cx(5)R phosphatase catalytic motif and 2 transmembrane domains near its C terminus, a characteristic of lysosomal transmembrane proteins. RNA dot blot analysis detected expression in all tissues examined. Fluorescence-tagged TMEM55A localized with membrane markers of late endosomes or lysosomes.Ungewickell et al. (2005) assayed the phosphatase activity of TMEM55A synthesized in insect cells and found that it specifically catalyzed removal of the 4-phosphate from PtdIns-4,5-P2. It showed no activity toward other phosphatidylinositol substrates or inositol phosphates tested.
Background: TMEM55A catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate.The deduced 300-amino acid protein contains a central Cx(5)R phosphatase catalytic motif and 2 transmembrane domains near its C terminus, a characteristic of lysosomal transmembrane proteins. RNA dot blot analysis detected expression in all tissues examined. Fluorescence-tagged TMEM55A localized with membrane markers of late endosomes or lysosomes.Ungewickell et al. (2005) assayed the phosphatase activity of TMEM55A synthesized in insect cells and found that it specifically catalyzed removal of the 4-phosphate from PtdIns-4,5-P2. It showed no activity toward other phosphatidylinositol substrates or inositol phosphates tested.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 4-phosphatase 2
NCBI Official Symbol
PIP4P2
NCBI Official Synonym Symbols
TMEM55A
NCBI Protein Information
type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase
UniProt Protein Name
Type 2 phosphatidylinositol 4,5-bisphosphate 4-phosphatase
UniProt Gene Name
TMEM55A
UniProt Synonym Gene Names
Type 2 PtdIns-4,5-P2 4-Ptase
UniProt Entry Name
TM55A_HUMAN
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Product Notes
The Human TMEM55A tmem55a (Catalog #AAA330497) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330497 ELISA Kit recognizes Human TMEM55A. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 55A (TMEM55A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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