Standard Curve (Sample)
(Fig.1. Human Transportin-1 (TNPO1) Standard Curve.)
Human Transportin-1 (TNPO1) ELISA Kit | TNPO1 elisa kit
Human Transportin-1 (TNPO1) ELISA Kit
Gene Names
TNPO1; MIP; TRN; IPO2; MIP1; KPNB2
Reactivity
Human
Synonyms
Transportin-1 (TNPO1); N/A; Human Transportin-1 (TNPO1) ELISA Kit; TNPO1; IPO2; KPNB2; MIP; MIP1; TRN; M9 region interaction protein; importin 2; importin beta 2; karyopherin (importin) beta 2; TNPO1 elisa kit
Reactivity
Human
Specificity
Human Transportin-1 (TNPO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNPO1. No significant cross-reactivity or interference between Human TNPO1 and analogues was observed.
Sequence Length
898
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Transportin-1 (TNPO1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Transportin-1 (TNPO1) Standard Curve.)
Related Product Information for TNPO1 elisa kit
Description: This Human Transportin-1 (TNPO1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNPO1 in samples. An antibody specific for TNPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNPO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Targeting of most nuclear proteins to the cell nucleus is initiated by interaction between the protein's nuclear localization signal (NLS) and the importin, or karyopherin, receptor complex. An importin heterodimer recognizes the NLS protein in the cytoplasm via its alpha subunit and, via its beta subunit, docks the complex to a subset of peptide repeat-containing proteins known as nucleoporins. Transportin-1 is the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins.
Background: Targeting of most nuclear proteins to the cell nucleus is initiated by interaction between the protein's nuclear localization signal (NLS) and the importin, or karyopherin, receptor complex. An importin heterodimer recognizes the NLS protein in the cytoplasm via its alpha subunit and, via its beta subunit, docks the complex to a subset of peptide repeat-containing proteins known as nucleoporins. Transportin-1 is the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transportin-1 isoform 1
NCBI Official Synonym Full Names
transportin 1
NCBI Official Symbol
TNPO1
NCBI Official Synonym Symbols
MIP; TRN; IPO2; MIP1; KPNB2
NCBI Protein Information
transportin-1
UniProt Protein Name
Transportin-1
UniProt Gene Name
TNPO1
UniProt Synonym Gene Names
KPNB2; MIP1; TRN; MIP
UniProt Entry Name
TNPO1_HUMAN
Similar Products
Product Notes
The Human TNPO1 tnpo1 (Catalog #AAA330404) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330404 ELISA Kit recognizes Human TNPO1. It is sometimes possible for the material contained within the vial of "Transportin-1 (TNPO1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.