Standard Curve (Sample)
(Fig.1. Human Trimethyllysine dioxygenase, mitochondrial (TMLHE) Standard Curve.)
Human Trimethyllysine Dioxygenase, Mitochondrial (TMLHE) ELISA Kit | TMLHE elisa kit
Human Trimethyllysine Dioxygenase, Mitochondrial (TMLHE) ELISA Kit
Gene Names
TMLHE; TMLD; TMLH; BBOX2; AUTSX6; TMLHED; XAP130
Reactivity
Human
Synonyms
Trimethyllysine Dioxygenase, Mitochondrial (TMLHE); N/A; Human Trimethyllysine Dioxygenase, Mitochondrial (TMLHE) ELISA Kit; TMLHE; BBOX2; FLJ10727; TMLH; XAP130; butyrobetaine (gamma); 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2; epsilon-trimethyllysine 2-oxoglutarate; epsilon-trimethyllysine hydroxylase; TMLHE elisa kit
Reactivity
Human
Specificity
Human Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMLHE. No significant cross-reactivity or interference between Human TMLHE and analogues was observed.
Sequence Length
421
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Trimethyllysine dioxygenase, mitochondrial (TMLHE) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Trimethyllysine dioxygenase, mitochondrial (TMLHE) Standard Curve.)
Related Product Information for TMLHE elisa kit
Description: This Human Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit employs a two-site sandwich ELISA to quantitate TMLHE in samples. An antibody specific for TMLHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMLHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMLHE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMLHE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Epsilon-N-trimethyllysine hydroxylase (EC 1.14.11.8) catalyzes the conversion of epsilon-N-trimethyllysine to beta-hydroxy-N-epsilon-trimethyllysine in the first step of L-carnitine biosynthesis. The deduced 421-amino acid human TMLHE and 405-amino acid rat Tmlhe proteins have calculated molecular masses of 49.5 kD and 47.5 kD, respectively, and share 92% amino acid identity. Subcellular fractionation localized rat Tmlhe to mitochondria. Rat Tmlhe was synthesized as a 47.5-kD precursor that is processed to an approximately 43-kD protein, most likely through cleavage of a mitochondrial targeting signal. Gel filtration analysis showed rat Tmlhe as an 87-kD band, suggesting that Tmlhe forms a homodimer.
Background: Epsilon-N-trimethyllysine hydroxylase (EC 1.14.11.8) catalyzes the conversion of epsilon-N-trimethyllysine to beta-hydroxy-N-epsilon-trimethyllysine in the first step of L-carnitine biosynthesis. The deduced 421-amino acid human TMLHE and 405-amino acid rat Tmlhe proteins have calculated molecular masses of 49.5 kD and 47.5 kD, respectively, and share 92% amino acid identity. Subcellular fractionation localized rat Tmlhe to mitochondria. Rat Tmlhe was synthesized as a 47.5-kD precursor that is processed to an approximately 43-kD protein, most likely through cleavage of a mitochondrial targeting signal. Gel filtration analysis showed rat Tmlhe as an 87-kD band, suggesting that Tmlhe forms a homodimer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Trimethyllysine dioxygenase, mitochondrial
NCBI Official Synonym Full Names
trimethyllysine hydroxylase, epsilon
NCBI Official Symbol
TMLHE
NCBI Official Synonym Symbols
TMLD; TMLH; BBOX2; AUTSX6; TMLHED; XAP130
NCBI Protein Information
trimethyllysine dioxygenase, mitochondrial
UniProt Protein Name
Trimethyllysine dioxygenase, mitochondrial
UniProt Gene Name
TMLHE
UniProt Synonym Gene Names
TMLH; TML dioxygenase; TMLD
UniProt Entry Name
TMLH_HUMAN
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Product Notes
The Human TMLHE tmlhe (Catalog #AAA330477) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330477 ELISA Kit recognizes Human TMLHE. It is sometimes possible for the material contained within the vial of "Trimethyllysine Dioxygenase, Mitochondrial (TMLHE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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