Human TRPA1 (Transient receptor potential cation channel subfamily A member 1) ELISA Kit | TRPA1 elisa kit

Human TRPA1 (Transient receptor potential cation channel subfamily A member 1) ELISA Kit

Cat. Num. AAA27591

• Gene Names: TRPA1; FEPS; FEPS1; ANKTM1
• Reactivity: Human
• Synonyms: TRPA1 (Transient receptor potential cation channel subfamily A member 1); N/A; Human TRPA1 (Transient receptor potential cation channel subfamily A member 1) ELISA Kit; TRPA1 elisa kit

• Reactivity: Human
• Specificity: Specifically recognize TRPA1, no obvious cross reaction with other analogues
• Sequence Length: 1119
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for TRPA1 elisa kit

Background: ANKTM1, also designated TRPA1, plays a role in both nociceptor and hair cell transduction. Activation of ANKTM1 occurs by perception of noxious cold (less than 17 degree C), and pungent natural compounds, such as garlic, cinnamon oil and mustard oil. Inhibition of ANKTM1 impairs hair cell mechanotransduction. Blocking ANKTM1 may be a therapeutic target for treating cold hyperalgesia caused by inflammation and nerve damage.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TRPA1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TRPA1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TRPA1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TRPA1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 116534990
• NCBI GeneID: 8989
• NCBI Accession #: NP_015628.2
• NCBI GenBank Nucleotide #: NM_007332.2
• UniProt Accession #: O75762; A6NIN6
• Molecular Weight: 127,501 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily A member 1
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily A member 1
• NCBI Official Symbol: TRPA1
• NCBI Official Synonym Symbols: FEPS; FEPS1; ANKTM1
• NCBI Protein Information: transient receptor potential cation channel subfamily A member 1
• UniProt Protein Name: Transient receptor potential cation channel subfamily A member 1
• UniProt Gene Name: TRPA1
• UniProt Synonym Gene Names: ANKTM1