Standard Curve (Sample)
Human Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit | TPPP elisa kit
Human Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit
Gene Names
TPPP; p24; p25; TPPP1; TPPP/p25; p25alpha
Reactivity
Human
Synonyms
Tubulin Polymerization Promoting Protein (TPPP); N/A; Human Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit; TPPP1; p25alpha; p25; TPPP/p25; Brain Specific Protein p25 Alpha; 25 kDa brain-specific protein; TPPP elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TPPP. No significant cross-reactivity or interference between TPPP and analogues was observed.
Sequence Length
219
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.132ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TPPP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TPPP elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TPPP in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPPP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TPPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TPPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TPPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPPP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TPPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TPPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TPPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,694 Da
NCBI Official Full Name
tubulin polymerization-promoting protein
NCBI Official Synonym Full Names
tubulin polymerization promoting protein
NCBI Official Symbol
TPPP
NCBI Official Synonym Symbols
p24; p25; TPPP1; TPPP/p25; p25alpha
NCBI Protein Information
tubulin polymerization-promoting protein
UniProt Protein Name
Tubulin polymerization-promoting protein
UniProt Gene Name
TPPP
UniProt Synonym Gene Names
TPPP1; TPPP
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Product Notes
The Human TPPP tppp (Catalog #AAA66835) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA66835 ELISA Kit recognizes Human TPPP. It is sometimes possible for the material contained within the vial of "Tubulin Polymerization Promoting Protein (TPPP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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