Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit | TNFSF9 elisa kit

Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit

Cat. Num. AAA13963

• Gene Names: TNFSF9; CD137L; TNLG5A; 4-1BB-L
• Reactivity: Human
• Synonyms: Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9); N/A; Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit; 4-1BB-L; Receptor 4-1BB Ligand; Homolog Of Mouse 4-1BB-L; TNFSF9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNFSF9. No significant cross-reactivity or interference between TNFSF9 and analogues was observed.
• Sequence Length: 254
• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.62-1000pg/mL
• Sensitivity: 5.35pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFSF9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFSF9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for TNFSF9 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFSF9 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFSF9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TNFSF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFSF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFSF9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507609
• NCBI GeneID: 8744
• NCBI Accession #: NP_003802.1
• NCBI GenBank Nucleotide #: NM_003811.3
• UniProt Accession #: P41273; Q2M3S2
• Molecular Weight: 26,625 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 9
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 9
• NCBI Official Symbol: TNFSF9
• NCBI Official Synonym Symbols: CD137L; TNLG5A; 4-1BB-L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 9
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 9
• UniProt Gene Name: TNFSF9
• UniProt Synonym Gene Names: 4-1BBL