Standard Curve (Sample)
(Fig.1. Human Tumor suppressor p53-binding protein 1 (TP53BP1) Standard Curve.)
Human Tumor Suppressor p53-Binding Protein 1 (TP53BP1) ELISA Kit | TP53BP1 elisa kit
Human Tumor Suppressor p53-Binding Protein 1 (TP53BP1) ELISA Kit
Gene Names
TP53BP1; p202; 53BP1; TDRD30; p53BP1
Reactivity
Human
Synonyms
Tumor Suppressor p53-Binding Protein 1 (TP53BP1); N/A; Human Tumor Suppressor p53-Binding Protein 1 (TP53BP1) ELISA Kit; TP53BP1; 53BP1; FLJ41424; MGC138366; p202; tumor protein 53-binding protein; 1; tumor protein p53-binding protein; TP53BP1 elisa kit
Reactivity
Human
Specificity
Human Tumor suppressor p53-binding protein 1 (TP53BP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TP53BP1. No significant cross-reactivity or interference between Human TP53BP1 and analogues was observed.
Sequence Length
1975
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Tumor suppressor p53-binding protein 1 (TP53BP1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Tumor suppressor p53-binding protein 1 (TP53BP1) Standard Curve.)
Related Product Information for TP53BP1 elisa kit
Description: This Human Tumor suppressor p53-binding protein 1 (TP53BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TP53BP1 in samples. An antibody specific for TP53BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TP53BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP53BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP53BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TP53BP1 has a predicted molecular mass of 217 kD and shows homology in its C-terminal 247 amino acids to the C terminus of the tumor suppressor protein BRCA1. TP53BP1 was present in both the cytoplasm and nucleus in some cells and only in the nucleus in others. Furthermore, there were 2 nuclear staining patterns, one homogeneous staining and the other dot staining.TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. TP53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins BRCA1 and CHK2 but was required for the formation of BRCA1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.
Background: TP53BP1 has a predicted molecular mass of 217 kD and shows homology in its C-terminal 247 amino acids to the C terminus of the tumor suppressor protein BRCA1. TP53BP1 was present in both the cytoplasm and nucleus in some cells and only in the nucleus in others. Furthermore, there were 2 nuclear staining patterns, one homogeneous staining and the other dot staining.TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. TP53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins BRCA1 and CHK2 but was required for the formation of BRCA1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
TP53-binding protein 1 isoform 2
NCBI Official Synonym Full Names
tumor protein p53 binding protein 1
NCBI Official Symbol
TP53BP1
NCBI Official Synonym Symbols
p202; 53BP1; TDRD30; p53BP1
NCBI Protein Information
TP53-binding protein 1
UniProt Protein Name
Tumor suppressor p53-binding protein 1
UniProt Gene Name
TP53BP1
UniProt Synonym Gene Names
53BP1; p53-binding protein 1; p53BP1
UniProt Entry Name
TP53B_HUMAN
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Product Notes
The Human TP53BP1 tp53bp1 (Catalog #AAA330377) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330377 ELISA Kit recognizes Human TP53BP1. It is sometimes possible for the material contained within the vial of "Tumor Suppressor p53-Binding Protein 1 (TP53BP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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