Standard Curve (Sample)
(Fig.1. Human Twisted gastrulation protein homolog 1 (TWSG1) Standard Curve.)
Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit | TWSG1 elisa kit
Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit
Gene Names
TWSG1; TSG
Reactivity
Human
Synonyms
Twisted Gastrulation Protein Homolog 1 (TWSG1); N/A; Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit; TWSG1; TSG; twisted gastrulation; TWSG1 elisa kit
Reactivity
Human
Specificity
Human Twisted gastrulation protein homolog 1 (TWSG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TWSG1. No significant cross-reactivity or interference between Human TWSG1 and analogues was observed.
Sequence Length
223
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Twisted gastrulation protein homolog 1 (TWSG1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Twisted gastrulation protein homolog 1 (TWSG1) Standard Curve.)
Related Product Information for TWSG1 elisa kit
Description: This Human Twisted gastrulation protein homolog 1 (TWSG1) ELISA Kit employs a two-site sandwich ELISA to quantitate TWSG1 in samples. An antibody specific for TWSG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TWSG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TWSG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TWSG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The cDNA encodes a protein sharing 41% amino acid identity with Drosophila Tsg, 89% identity with the partial human Tsg sequence, and 94% identity with a mouse EST. The Tsg sequence contains a signal peptide, as expected for a secreted protein, and 2 conserved domains containing multiple cysteines at its amino and carboxy termini. At the late gastrula stage, maternal transcripts decrease and zygotic transcripts appear, specifically in the ventral region of the embryo. Tsg has ventralizing activity and is a bone morphogenetic protein (BMP) binding protein. The N-terminal domain of Tsg is sufficient to interact with BMP4 but not with chordin. Tsg competes for binding of BMP4 with the first cysteine-rich domain of chordin (CR1) but not with full-length chordin. Endogenous Tsg antagonizes CR1 activity.
Background: The cDNA encodes a protein sharing 41% amino acid identity with Drosophila Tsg, 89% identity with the partial human Tsg sequence, and 94% identity with a mouse EST. The Tsg sequence contains a signal peptide, as expected for a secreted protein, and 2 conserved domains containing multiple cysteines at its amino and carboxy termini. At the late gastrula stage, maternal transcripts decrease and zygotic transcripts appear, specifically in the ventral region of the embryo. Tsg has ventralizing activity and is a bone morphogenetic protein (BMP) binding protein. The N-terminal domain of Tsg is sufficient to interact with BMP4 but not with chordin. Tsg competes for binding of BMP4 with the first cysteine-rich domain of chordin (CR1) but not with full-length chordin. Endogenous Tsg antagonizes CR1 activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
twisted gastrulation protein homolog 1
NCBI Official Synonym Full Names
twisted gastrulation BMP signaling modulator 1
NCBI Official Symbol
TWSG1
NCBI Official Synonym Symbols
TSG
NCBI Protein Information
twisted gastrulation protein homolog 1
UniProt Protein Name
Twisted gastrulation protein homolog 1
UniProt Gene Name
TWSG1
UniProt Synonym Gene Names
TSG
UniProt Entry Name
TWSG1_HUMAN
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Product Notes
The Human TWSG1 twsg1 (Catalog #AAA330338) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330338 ELISA Kit recognizes Human TWSG1. It is sometimes possible for the material contained within the vial of "Twisted Gastrulation Protein Homolog 1 (TWSG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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