Standard Curve (Sample)
(Fig.1. Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) Standard Curve.)
Human UbiA Prenyltransferase Domain-Containing Protein 1 (UBIAD1) ELISA Kit | UBIAD1 elisa kit
Human UbiA Prenyltransferase Domain-Containing Protein 1 (UBIAD1) ELISA Kit
Gene Names
UBIAD1; SCCD; TERE1
Reactivity
Human
Synonyms
UbiA Prenyltransferase Domain-Containing Protein 1 (UBIAD1); N/A; Human UbiA Prenyltransferase Domain-Containing Protein 1 (UBIAD1) ELISA Kit; UBIAD1; RP4-796F18.1; SCCD; TERE1; transitional epithelia response protein; UBIAD1 elisa kit
Reactivity
Human
Specificity
Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBIAD1. No significant cross-reactivity or interference between Human UBIAD1 and analogues was observed.
Sequence Length
338
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) Standard Curve.)
Related Product Information for UBIAD1 elisa kit
Description: This Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate UBIAD1 in samples. An antibody specific for UBIAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBIAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBIAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBIAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TERE1 expression in both superficial and muscle-invasive transitional cell carcinomas of bladder (TCCs) compared with normal human urothelium. Transfection of TERE1 into 2 human TCC cell lines inhibited cellular proliferation by 80 to 90%. In 1 line, TERE1 overexpression caused a change in morphology, with an apparent increase in intercellular contacts The deduced 338-amino acid protein has a calculated molecular mass of 36.8 kD. TERE1 has 5 putative transmembrane regions, an N-glycosylation site, and several putative phosphorylation sites. Northern blot and PCR analyses showed ubiquitous expression of 1.5- and 3-kb TERE1 transcripts. Immunohistochemical analysis of human urothelium revealed nuclear and cytoplasmic expression of TERE1.
Background: TERE1 expression in both superficial and muscle-invasive transitional cell carcinomas of bladder (TCCs) compared with normal human urothelium. Transfection of TERE1 into 2 human TCC cell lines inhibited cellular proliferation by 80 to 90%. In 1 line, TERE1 overexpression caused a change in morphology, with an apparent increase in intercellular contacts The deduced 338-amino acid protein has a calculated molecular mass of 36.8 kD. TERE1 has 5 putative transmembrane regions, an N-glycosylation site, and several putative phosphorylation sites. Northern blot and PCR analyses showed ubiquitous expression of 1.5- and 3-kb TERE1 transcripts. Immunohistochemical analysis of human urothelium revealed nuclear and cytoplasmic expression of TERE1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiA prenyltransferase domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
UbiA prenyltransferase domain containing 1
NCBI Official Symbol
UBIAD1
NCBI Official Synonym Symbols
SCCD; TERE1
NCBI Protein Information
ubiA prenyltransferase domain-containing protein 1
UniProt Protein Name
UbiA prenyltransferase domain-containing protein 1
UniProt Gene Name
UBIAD1
UniProt Synonym Gene Names
TERE1
UniProt Entry Name
UBIA1_HUMAN
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Product Notes
The Human UBIAD1 ubiad1 (Catalog #AAA330321) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330321 ELISA Kit recognizes Human UBIAD1. It is sometimes possible for the material contained within the vial of "UbiA Prenyltransferase Domain-Containing Protein 1 (UBIAD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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